| Author | Camaforte, Nathalia A. P. de Paula | |
| Author | Saldanha, Luiz Leonardo | |
| Author | Vareda, Priscilla Maria Ponce | |
| Author | Rezende Neto, João M. | |
| Author | Senger, Mario R. | |
| Author | Delgado, Aislan Q. | |
| Author | Morgan, Henrique J. N. | |
| Author | Violato, Natalia Moretti | |
| Author | Pieroni, Laís Goyos | |
| Author | Dokkedal, Anne Lígia | |
| Author | Silva Júnior, Floriano P. | |
| Author | Bosqueiro, José Roberto | |
| Access date | 2019-09-03T16:32:27Z | |
| Available date | 2019-09-03T16:32:27Z | |
| Document date | 2019 | |
| Citation | CAMAFORTE, Nathalia A. P. de Paula et al. Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation. Pharmaceutical Biology, v. 57, n. 1, p. 269-279, 2019. | pt_BR |
| ISSN | 1388-0209 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/35318 | |
| Language | eng | pt_BR |
| Publisher | Taylor & Francis Open | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Antidiabético | pt_BR |
| Subject in Portuguese | Flavonóides-O-glicosídeos | pt_BR |
| Subject in Portuguese | Fígado | pt_BR |
| Subject in Portuguese | Lipídios plasmáticos | pt_BR |
| Subject in Portuguese | Fabaceae | pt_BR |
| Title | Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation | pt_BR |
| Type | Article | |
| DOI | 10.1080/13880209.2019.1599962 | |
| Abstract | Context: Bauhinia L. species, including Bauhinia holophylla (Bong.) Steud. (Fabaceae), have traditionally been used to treat diabetes. Bauhinia is a complex botanical genus, and the indiscriminate use of the diverse Bauhinia species is reflected in the experimental divergence of their medicinal potential. Objective: The hypoglycaemic and hypolipidaemic effects, molecular mechanism of action and phytochemical properties of an authentic extract of B. holophylla leaves were evaluated. Materials and methods: A phytochemical study of a 70% EtOH extract was performed using FIA-ESI-ITMS/MSn and HPLC-PAD-ESI-IT-MS. The extract (200 or 400 mg/kg b.w.) was administered for 14 days to streptozotocin-induced diabetic Swiss mice. Glucose tolerance and insulin sensitivity, blood parameters, gene and protein expression, and the in vivo and in vitro inhibition of intestinal glucosidases were assessed. Results: HPLC-PAD-ESI-IT-MS analysis identified flavonoid derivatives of quercetin, myricetin, luteolin and kaempferol. Treatment with 400 mg/kg of the extract reduced blood glucose (269.0 ± 32.4 mg/dL vs. 468.0 ± 32.2 mg/dL for diabetic animals), improved glucose tolerance, decreased cholesterol and triglyceride levels, and increased the mRNA expression of proteins involved in glucogenesis in the liver and muscle, such as PI3-K/Akt, GS, GSK3-b (ser-9), AMPK and Glut4. The activity of intestinal maltase was inhibited in vitro (IC50: 43.0 mg/mL for the extract compared to 516.4 mg/mL for acarbose) and in vivo. Discussion and conclusions: Treatment with B. holophylla was associated with a marked hypoglycaemic effect through the stimulation of glycogenesis and inhibition of gluconeogenesis and intestinal glucose absorption, without increasing basal insulinaemia. | pt_BR |
| Affilliation | Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Drogas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Drogas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Universidade do Estado de São Paulo. Departamento de Ciências Biológicas. Bauru, SP, Brasil. | pt_BR |
| Affilliation | Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Universidade do Estado de São Paulo. Departamento de Ciências Biológicas. Bauru, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Drogas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado de São Paulo. Departamento de Educação Física. Bauru, SP, Brasil. | pt_BR |
| Subject | Antidiabetic | pt_BR |
| Subject | Fabaceae | pt_BR |
| Subject | Flavonoid-O-glycosides | pt_BR |
| Subject | Liver | pt_BR |
| Subject | Plasma lipids | pt_BR |
| e-ISSN | 1744-5116 | |
