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DUAL DNA BARCODING FOR THE MOLECULAR IDENTIFICATION OF THE AGENTS OF INVASIVE FUNGAL INFECTIONS
Fungal DNA barcoding
Dual barcoding system
Internal transcribed spacer region
Translational elongation factor 1α
ISHAM Barcoding Database
Invasive fungal diseases
Autor
Afiliación
University of Sydney. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Westmead Institute for Medical Research. Westmead, NSW, Australia.
University of Sydney. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Westmead Institute for Medical Research. Westmead, NSW, Australia / Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney, NSW, Australia.
University of Sydney. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney, NSW, Australia / NSW Health Pathology. Institute for Clinical Pathology and Medical Research. Centre for Infectious Diseases and Microbiology Laboratory Services. Westmead, NSW, Australia.
University of Sydney. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Westmead Institute for Medical Research. Westmead, NSW, Australia / Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney, NSW, Australia.
University of Sydney. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Westmead Institute for Medical Research. Westmead, NSW, Australia / Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney, NSW, Australia / Westmead Hospital. Research and Education Network. Westmead, NSW, Australia.
University of Sydney. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Westmead Institute for Medical Research. Westmead, NSW, Australia / Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney, NSW, Australia.
University of Sydney. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney, NSW, Australia / NSW Health Pathology. Institute for Clinical Pathology and Medical Research. Centre for Infectious Diseases and Microbiology Laboratory Services. Westmead, NSW, Australia.
University of Sydney. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Westmead Institute for Medical Research. Westmead, NSW, Australia / Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney, NSW, Australia.
University of Sydney. Faculty of Medicine and Health. Sydney Medical School. Westmead Clinical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / Westmead Institute for Medical Research. Westmead, NSW, Australia / Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney, NSW, Australia / Westmead Hospital. Research and Education Network. Westmead, NSW, Australia.
Resumen en ingles
Invasive fungal infections, such as aspergillosis, candidiasis, and cryptococcosis, have significantly increased among immunocompromised people. To tackle these infections the first and most decisive step is the accurate identification of the causal pathogen. Routine identification of invasive fungal infections has progressed away from culture-dependent methods toward molecular techniques, including DNA barcoding, a highly efficient and widely used diagnostic technique. Fungal DNA barcoding previously relied on a single barcoding region, the internal transcribed spacer (ITS) region. However, this allowed only for 75% of all fungi to be correctly identified. As such, the translational elongation factor 1α (TEF1α) was recently introduced as the secondary barcode region to close the gap. Both loci together form the dual fungal DNA barcoding scheme. As a result, the ISHAM Barcoding Database has been expanded to include sequences for both barcoding regions to enable practical implementation of the dual barcoding scheme into clinical practice. The present study investigates the impact of the secondary barcode on the identification of clinically important fungal taxa, that have been demonstrated to cause severe invasive disease. Analysis of the barcoding regions was performed using barcoding gap analysis based on the genetic distances generated with the Kimura 2-parameter model. The secondary barcode demonstrated an improvement in identification for all taxa that were unidentifiable with the primary barcode, and when combined with the primary barcode ensured accurate identification for all taxa analyzed, making DNA barcoding an important, efficient and reliable addition to the diagnostic toolset of invasive fungal infections.
Palabras clave en ingles
IdentificationFungal DNA barcoding
Dual barcoding system
Internal transcribed spacer region
Translational elongation factor 1α
ISHAM Barcoding Database
Invasive fungal diseases
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