| Author | Torres, E. J. Lopes | |
| Author | Dias, W. Girard | |
| Author | Mello, W. N. | |
| Author | Simões, R. O. | |
| Author | Pinto, I. S. | |
| Author | Maldonado, A. | |
| Author | Souza, W. de | |
| Author | Miranda, K. | |
| Access date | 2019-09-17T17:07:19Z | |
| Available date | 2019-09-17T17:07:19Z | |
| Document date | 2019 | |
| Citation | TORRES, E. J. Lopes et al. Taxonomy of Physaloptera mirandai (Nematoda: Physalopteroidea) based in three-dimensional microscopy and phylogenetic positioning. Acta Tropica, v. 195, p. 115-126, Apr. 2019. | pt_BR |
| ISSN | 0001-706X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/35646 | |
| Language | eng | pt_BR |
| Publisher | Elsevier 12 Months | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Taxonomia | pt_BR |
| Subject in Portuguese | Nematóide | pt_BR |
| Subject in Portuguese | Filogenia | pt_BR |
| Subject in Portuguese | Microscópio eletrônic | pt_BR |
| Subject in Portuguese | Modelagem 3d | pt_BR |
| Subject in Portuguese | Physaloptera | pt_BR |
| Title | Taxonomy of Physaloptera mirandai (Nematoda: Physalopteroidea) based in three-dimensional microscopy and phylogenetic positioning | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.actatropica.2019.04.002 | |
| Abstract | Gastrointestinal nematodes are important ecological assets for the maintenance of the biodiversity in the Atlantic Forest in Brazil. They parasitize a number of animals of the local fauna, in which some species can promote serious injuries in the stomach wall of their hosts, which may lead to death. Among these nematodes, parasites of the genus Physaloptera are known to parasitize mammals (particularly carnivores and small rodents), birds and reptiles, being important for the local biodiversity. In this work, three hundred and sixty-two nematodes were recovered from the stomach of twenty-one Metachirus nudicaudatus (Didelphimorphia: Didelphidae) collected in Duas Bocas Biological Reserve, State of Espírito Santo, one of the largest Atlantic Forest remnants and important wildlife refuge of the Atlantic Forest in Brazil. Analysis using fluorescence and scanning electron microscopy as well as phylogenetic assessment using the mitochondrial cytochrome c oxidase subunit I gene showed that the parasites belong to the Physaloptera. Our results show details of the nematode morphology including the cloacal papillae distribution, cuticular topography details, 2D and 3D measurements of the structures with taxonomic importance. Molecular data confirmed the validity of P. mirandai and the phylogeny supported the monophyly of the assemblage formed by Physaloptera and Turgida. The use of a combination of quantitative and multidimensional microscopy tools, such as 3D reconstruction and modeling, allied to phylogenetic analysis may provide grounds for a new approach on helminth taxonomy and structural characterization. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Romero Lascasas Porto. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Romero Lascasas Porto. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Laboratório de Parasitologia. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Universidade Federal Rural do Rio de Janeiro. Departamento de Parasitologia Animal. Seropédica, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Biologia Estrutural e Bioimagem. Centro Nacional de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Biologia Estrutural e Bioimagem. Centro Nacional de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Taxonomy | pt_BR |
| Subject | Nematode | pt_BR |
| Subject | Phylogeny | pt_BR |
| Subject | Physaloptera | pt_BR |
| Subject | Electron microscopy | pt_BR |
| Subject | 3D modeling | pt_BR |
| e-ISSN | 1873-6254 | |
| Embargo date | 2025-01-01 | |
