| Author | Rodríguez-Arellanes, Gabriela | |
| Author | Sousa, Carolina Nascimento de | |
| Author | Medeiros-Muniz, Mauro de | |
| Author | Ramírez, José A. | |
| Author | Pizzini, Cláudia V. | |
| Author | Abreu-Almeida, Marcos de | |
| Author | Oliveira, Manoel M. Evangelista de | |
| Author | Fusco-Almeida, Ana-Marisa | |
| Author | Vite-Garín, Tania | |
| Author | Pitangui, Nayla S. | |
| Author | Estrada-Bárcenas, Daniel A. | |
| Author | González-González, Antonio E. | |
| Author | Mendes-Giannini, María José Soares | |
| Author | Zancopé-Oliveira, Rosely M. | |
| Author | Taylor, Maria-Lucia | |
| Access date | 2019-09-19T13:24:06Z | |
| Available date | 2019-09-19T13:24:06Z | |
| Document date | 2013 | |
| Citation | RODRÍGUEZ-ARELLANA, Gabriela et al. Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil. Eukaryotic Cell, v. 12, n. 7, p. 1033-1038, 2013. | pt_BR |
| ISSN | 1535-9778 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/35677 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | pt_BR |
| Title | Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1128/EC.00012-13 | |
| Abstract | The MAT1-1 and MAT1-2 idiomorphs associated with the MAT1 locus of Histoplasma capsulatum were identified by PCR. A total of 28 fungal isolates, 6 isolates from human clinical samples and 22 isolates from environmental (infected bat and contaminated soil) samples, were studied. Among the 14 isolates from Mexico, 71.4% (95% confidence interval [95% CI], 48.3% to 94.5%) were of the MAT1-2 genotype, whereas 100% of the isolates from Brazil were of the MAT1-1 genotype. Each MAT1 idiomorphic region was sequenced and aligned, using the sequences of the G-217B (+ mating type) and G-186AR (- mating type) strains as references. BLASTn analyses of the MAT1-1 and MAT1-2 sequences studied correlated with their respective + and - mating type genotypes. Trees were generated by the maximum likelihood (ML) method to search for similarity among isolates of each MAT1 idiomorph. All MAT1-1 isolates originated from Brazilian bats formed a well-defined group; three isolates from Mexico, the G-217B strain, and a subgroup encompassing all soil-derived isolates and two clinical isolates from Brazil formed a second group; last, one isolate (EH-696P) from a migratory bat captured in Mexico formed a third group of the MAT1-1 genotype. The MAT1-2 idiomorph formed two groups, one of which included two H. capsulatum isolates from infected bats that were closely related to the G-186AR strain. The other group was formed by two human isolates and six isolates from infected bats. Concatenated ML trees, with internal transcribed spacer 1 (ITS1) -5.8S-ITS2 and MAT1-1 or MAT1-2 sequences, support the relatedness of MAT1-1 or MAT1-2 isolates. H. capsulatum mating types were associated with the geographical origin of the isolates, and all isolates from Brazil correlated with their environmental sources. | pt_BR |
| Affilliation | Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara, SP, Brasil. | pt_BR |
| Affilliation | Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara, SP, Brasil. | pt_BR |
| Affilliation | Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Colección Nacional de Cultivos Microbianos. México DF, Mexico. | pt_BR |
| Affilliation | Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico. | pt_BR |
| Subject | Histoplasma capsulatum | pt_BR |
| Subject | MAT1 | pt_BR |
| Subject | Mexico | pt_BR |
| Subject | Brazil | pt_BR |
| e-ISSN | 1535-9786 | |
| Embargo date | 2020-03-19 | |
