| Author | Bezerra, Gilberto Silva Nunes | |
| Author | Barbosa, Walter Lins | |
| Author | Silva, Elis Dionísio da | |
| Author | Leal, Nilma Cintra | |
| Author | Medeiros, Zulma Maria de | |
| Access date | 2019-09-26T12:17:57Z | |
| Available date | 2019-09-26T12:17:57Z | |
| Document date | 2019 | |
| Citation | BEZERRA, Gilberto Silva Nunes et al. Urine as a promising sample for Leishmania DNA extraction in the diagnosis of visceral leishmaniasis - a review. The Brazilian Journal of Infectious Diseases, v. 23, n. 2, p. 111–120, Apr. 2019. | pt_BR |
| ISSN | 1413-8670 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/35909 | |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Urine as a promising sample for Leishmania DNA extraction in the diagnosis of visceral leishmaniasis - a review | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.bjid.2019.04.001 | |
| Abstract | Visceral leishmaniasis is a serious and debilitating infection with high fatality rate in tropical and subtropical countries. As clinical symptoms of visceral leishmaniasis are not so specific, confirmatory diagnostic methods with high sensitivity and specificity are needed. Noninvasive methods have been developed using urine as a clinical sample for visceral leishmaniasis diagnosis. In fact, there is a clear correlation between kidney impairment and Leishmania DNA in urine. However, it has been proved that Leishmania nucleic acid may also be isolated from patients without any sign of renal involvement. Even though urine has become a promissing biological sample, it is still not widely used due to several issues, such as (i) incomprehension of the whole renal pathophysiology process in visceral leishmaniasis, (ii) presence of many amplification inhibitors in urine, and (iii) lack of an efficient urinary DNA extraction method. In this article, we performed a literature review to bring a new perspective for Leishmania DNA isolation in urine. | pt_BR |
| Affilliation | Universidade de Pernambuco. Programa de Pós-Graduação em Ciências da Saúde. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade de Pernambuco. Programa de Pós-Graduação em Ciências da Saúde. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil. | pt_BR |
| Subject | DNA extraction | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Renal involvement | pt_BR |
| Subject | Urine sample | pt_BR |
| Subject | Visceral leishmaniasis | pt_BR |
| DeCS | DNA de Protozoário | pt_BR |
| DeCS | Isolamento & purificação | pt_BR |
| DeCS | Urina | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Leishmania | pt_BR |
| DeCS | Genética | pt_BR |
| DeCS | Leishmaniose Visceral | pt_BR |
| DeCS | Diagnóstico | pt_BR |
| DeCS | Reação em Cadeia da Polimerase | pt_BR |
| DeCS | Métodos | pt_BR |
| DeCS | Reprodutibilidade dos Testes | pt_BR |
| DeCS | Sensibilidade e Especificidade | pt_BR |
| Embargo date | 2050-01-01 | |
