| Author | Nogueira, Nadir F. S. | |
| Author | Gonzalez, Marcelo S. | |
| Author | Gomes, José Eugênio | |
| Author | Souza, Wanderley de | |
| Author | Garcia, Eloi S. | |
| Author | Azambuja, Patricia | |
| Author | Nohara, Lilian L. | |
| Author | Almeida, Igor C. | |
| Author | Zingales, Bianca | |
| Author | Colli, Walter | |
| Access date | 2019-10-10T15:31:48Z | |
| Available date | 2019-10-10T15:31:48Z | |
| Document date | 2007 | |
| Citation | NOGUEIRA, Nadir F. S. et al. Trypanosoma cruzi: Involvement of glycoinositolphospholipids in the attachment to the luminal midgut surface of Rhodnius prolixus. Experimental Parasitology, v. 116, p. 120-128, 2007. | pt_BR |
| ISSN | 0014-4894 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/36344 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Espectrometria de massa de ionização por electropulverização | pt_BR |
| Subject in Portuguese | Membranas perimicrovilares | pt_BR |
| Subject in Portuguese | Rhodnius prolixus | pt_BR |
| Subject in Portuguese | Superfície intestinal | pt_BR |
| Title | Trypanosoma cruzi: involvement of glycoinositolphospholipids in the attachment to the luminal midgut surface of Rhodnius prolixus | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.exppara.2006.12.014 | |
| Abstract | Trypanosoma cruzi epimastigotes adhere in vivo to the luminal surface of their triatomid vector digestive tract by molecular mechanisms, as yet, unknown. Here, we show that the administration of 0.5 microM epimastigote major surface glycoinositolphospholipids (GIPLs) to the infected bloodmeal inhibits up to 90% parasite infection in Rhodnius prolixus. The parasite behavior was investigated in vitro using fragments of the insect midgut. The addition of GIPLs in concentration as low as 50-100 nM impaired 95% the attachment of epimastigotes. Previous treatment of GIPLs with trifluoroacetic acid to remove the terminal beta-galactofuranosyl residues reversed 50% the epimastigote in vitro attachment. The binding sites of purified GIPLs on the luminal surface of the posterior midgut were exposed by immunofluorescence microscopy. These observations indicate that GIPLs are one of the components involved in the adhesion of T. cruzi to the luminal insect midgut surface and possibly one of the determinants of parasite infection in the insect vector. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacazes, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of Texas at El Paso. Department of Biological Sciences. TX, USA / Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | University of Texas at El Paso. Department of Biological Sciences. TX, USA / Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Glycoinositolphospholipids | pt_BR |
| Subject | Electrospray ionization-mass spectrometry | pt_BR |
| Subject | perimicrovillar membranes | pt_BR |
| Subject | Rhodnius prolixus | pt_BR |
| Subject | Midgut surface | pt_BR |
| e-ISSN | 1090-2449 | |
