| Author | Garcia, Eloi S. | |
| Author | Castro, Daniele P. | |
| Author | Ribeiro, Ivone M. | |
| Author | Tomassini, Therezinha C. B. | |
| Author | Azambuja, Patricia | |
| Access date | 2019-10-10T16:00:38Z | |
| Available date | 2019-10-10T16:00:38Z | |
| Document date | 2006 | |
| Citation | GARCIA, Eloi S. et al. Trypanosoma rangeli: Effects of physalin B on the immune reactions of the infected larvae of Rhodnius prolixus. Experimental Parasitology, v. 112, p. 37-43, Nov. 2006. | pt_BR |
| ISSN | 0014-4894 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/36346 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Rhodnius prolixus | pt_BR |
| Subject in Portuguese | Trypanosoma rangeli | pt_BR |
| Subject in Portuguese | Hemócitos | pt_BR |
| Subject in Portuguese | Microagregação | pt_BR |
| Subject in Portuguese | Óxido nítrico | pt_BR |
| Title | Trypanosoma rangeli: Effects of physalin B on the immune reactions of the infected larvae of Rhodnius prolixus | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.exppara.2005.09.003 | |
| Abstract | Physalins are seco-steroids obtained from plants of the family Solanaceae. Herein, we tested Physalis angulata L puriWed physalin B as
an immunomodulatory compound in 5th-instar larvae of Rhodnius prolixus, which were systemically infected with the H14 Trypanosoma
rangeli strain protozoan. In uninfected insects, the eVective concentration of physalin B, which inhibited 50% of the blood ingested (ED50)
volume, was 15.2§1.6 g/ml of the meal. Ecdysis processes and mortality in uninfected larvae, treated orally with physalin B in concentrations
ranging from 1 to 10 g/ml, was similar to that observed in insects not treated with physalin B. However, R. prolixus larvae previously
fed on blood containing 1.0, 0.1, and 0.01 g of physalin B/ml exhibited mortality rates of 78.1, 54.3, and 12.7%, respectively, 6 days
after inoculation of T. rangeli (1£103 parasites/insect), whereas only 7.2% mortality was observed in the control group, injected with sterile
culture medium. The insects treated with physalin B (0.1 g/ml) and inoculated with T. rangeli did not modify the phenoloxidase (PO)
activity and total hemocyte count in the hemolymph. However, physalin B treatment caused a reduction in hemocyte microaggregation
and nitric oxide production and enhanced the parasitemia in the hemolymph. These results demonstrate that physalin B from P. angulata
is a potent immunomodulatory substance for the bloodsucking insect, R. prolixus. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Rhodnius prolixus | pt_BR |
| Subject | Seco-steroids | pt_BR |
| Subject | Physalis angulata L | pt_BR |
| Subject | Physalin | pt_BR |
| Subject | Trypanosoma rangeli | pt_BR |
| Subject | Hemocytes | pt_BR |
| Subject | Microaggregation | pt_BR |
| Subject | Nitric Oxide | pt_BR |
| Subject | Prophenoloxidase | pt_BR |
| e-ISSN | 1090-2449 | |
| Embargo date | 2022-01-01 | |
