Autor | Machado, Evandro M. M. | |
Autor | Azambuja, Patricia | |
Autor | Garcia, Eloi S. | |
Data de acesso | 2019-10-10T16:37:10Z | |
Data de disponibilização | 2019-10-10T16:37:10Z | |
Data do publicação | 2006 | |
Citação | MACHADO, Evandro M. M.; AZAMBUJA, Patricia; GARCIA, Eloi S. WEB 2086, a platelet-activating factor antagonist, inhibits prophenoloxidase-activating system and hemocyte microaggregation reactions induced by Trypanosoma rangeli infection in Rhodnius prolixus hemolymph. Journal of Insect Physiology, v. 52, p. 685–692, 2006. | pt_BR |
ISSN | 0022-1910 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/36349 | |
Idioma | eng | pt_BR |
Editor | Elsevier | pt_BR |
Direito Autoral | restricted access | pt_BR |
Palavras-chave | Trypanosoma rangeli | pt_BR |
Palavras-chave | Rhodnius prolixus | pt_BR |
Palavras-chave | Prophenoloxidase | pt_BR |
Palavras-chave | Trypanosoma rangeli | pt_BR |
Palavras-chave | Rhodnius prolixus | pt_BR |
Palavras-chave | Microagregação de hemócitos | pt_BR |
Título | WEB 2086, a platelet-activating factor antagonist, inhibits prophenoloxidase-activating system and hemocyte microaggregation reactions induced by Trypanosoma rangeli infection in Rhodnius prolixus hemolymph | pt_BR |
Tipo do documento | Article | pt_BR |
DOI | 10.1016/j.jinsphys.2006.03.008 | |
Resumo em Inglês | The effects of the triazolodiazepine WEB 2086, a platelet-activating factor (PAF) antagonist, on hemocyte microaggregation and prophenoloxidase (proPO)-activating system in the hemolymph, hemocoelic infection and mortality in fifth-instar larvae of Rhodnius prolixus inoculated with Trypanosoma rangeli were investigated. Hemocoelic injection of short T. rangeli epimastigotes (1 104 parasites/ insect) in R. prolixus that were previously fed with blood containing 1 mM of WEB 2086 resulted in (i) reduced hemocyte microaggregations as well as an attenuated proPO system in the hemolymph and (ii) greater parasitemia and mortality among the insects. In vitro assays using hemolymph from insects previously fed with blood containing WEB 2086 exhibited attenuated hemocyte microaggregations when T. rangeli was employed as the inducer of the reaction, and this effect was not counteracted by PAF treatment. In vitro assays using hemolymph from insects previously fed with blood, regardless of WEB 2086 presence increased the PO activity when incubated with the parasites. However, the PO activity was drastically inhibited when hemolymph from insects fed with blood, whether or not it contained WEB 2086, was incubated with fat body homogenates from insects fed with blood containing WEB 2086. The addition of PAF did not enhance the PO activity. These analyses did not reveal any PAF influence on WEB 2086 effects in the two defense reactions. | pt_BR |
Afiliação | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Afiliação | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Afiliação | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Palavras-chave em inglês | WEB 2086 | pt_BR |
Palavras-chave em inglês | PAF | pt_BR |
Palavras-chave em inglês | Hemocyte microaggregation | pt_BR |
Palavras-chave em inglês | Prophenoloxidase | pt_BR |
Palavras-chave em inglês | Trypanosoma rangeli | pt_BR |
Palavras-chave em inglês | Rhodnius prolixus | pt_BR |
DeCS | Fator de Ativação de Plaquetas | pt_BR |
e-ISSN | 1879-1611 | |
Data de embargo | 2022-01-01 | |