| Author | Moura, Danielle M. N. | |
| Author | Melo Neto, Osvaldo P. de | |
| Author | Carrington, Mark | |
| Access date | 2019-10-16T12:48:39Z | |
| Available date | 2019-10-16T12:48:39Z | |
| Document date | 2019 | |
| Citation | MOURA, Danielle M. N.; MELO NETO, Osvaldo P. de; CARRINGTON, Mark. A new reporter cell line for studies with proteasome inhibitors in Trypanosoma brucei. Molecular and Biochemical Parasitology, v. 227, p. 15-18, 2019. | pt_BR |
| ISSN | 0166-6851 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/36483 | |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Title | A new reporter cell line for studies with proteasome inhibitors in Trypanosoma brucei | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.molbiopara.2018.11.001 | |
| Abstract | A Trypanosoma brucei cell line is described that produces a visual readout of proteasome activity. The cell line contains an integrated transgene encoding an ubiquitin-green fluorescent protein (GFP) fusion polypeptide responsive to the addition of proteasome inhibitors. A modified version of T. brucei ubiquitin unable to be recognized by deubiquitinases (UbG76V) was fused to eGFP and constitutively expressed. The fusion protein is unstable but addition of the proteasome inhibitor lactacystin stabilizes it and leads to visually detectable GFP. This cell line can be widely used to monitor the efficiency of inhibitor treatment through detection of GFP accumulation in studies involving proteasome-mediated proteolysis, screening of proteasome inhibitors or other events related to the ubiquitin-proteasome pathway. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / University of Cambridge. Department of Biochemistry. Cambridge, United Kingdom. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | University of Cambridge. Department of Biochemistry. Cambridge, United Kingdom. | pt_BR |
| Subject | Lactacystin | pt_BR |
| Subject | Proteasome | pt_BR |
| Subject | Trypanosoma brucei | pt_BR |
| Subject | Ubiquitin | pt_BR |
| DeCS | Linhagem Celular | pt_BR |
| DeCS | Avaliação Pré-Clínica de Medicamentos / métodos | pt_BR |
| DeCS | Genes Reporter | pt_BR |
| DeCS | Proteínas de Fluorescência Verde / genética | pt_BR |
| DeCS | Proteínas de Fluorescência Verde / metabolismo | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Complexo de Endopeptidases do Proteassoma / genética | pt_BR |
| DeCS | Complexo de Endopeptidases do Proteassoma / metabolismo | pt_BR |
| DeCS | Inibidores de Proteassoma / farmacologia | pt_BR |
| DeCS | Tripanossomicidas / farmacologia | pt_BR |
| DeCS | Trypanosoma brucei brucei / efeitos dos fármacos | pt_BR |
| DeCS | Trypanosoma brucei brucei / enzimologia | pt_BR |
| DeCS | Trypanosoma brucei brucei / genética | pt_BR |
| DeCS | Tripanossomíase Africana / parasitologia | pt_BR |
| DeCS | Ubiquitina / metabolismo | pt_BR |
| Embargo date | 2050-01-01 | |
