| Author | Gonçales, Juliana Prado | |
| Author | Lopes, Thaísa Regina Rocha | |
| Author | Lorena, Virginia Maria Barros de | |
| Author | Aciole, Melayne Rocha | |
| Author | Silva Júnior, José Valter Joaquim | |
| Author | Tozetto-Mendoza, Tania Regina | |
| Author | Braz-Silva, Paulo Henrique | |
| Author | Coêlho, Maria Rosangela Cunha Duarte | |
| Access date | 2019-10-21T13:47:43Z | |
| Available date | 2019-10-21T13:47:43Z | |
| Document date | 2019 | |
| Citation | GONÇALES, Juliana Prado et al. Association of NFκB and related-cytokines with the viral load and development of antibodies against HHV-8 in people living with HIV/AIDS. Medical Microbiology and Immunology, p. 1-9, 4 Oct. 2019. | pt_BR |
| ISSN | 0300-8584 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/36598 | |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Association of NFκB and related-cytokines with the viral load and development of antibodies against HHV-8 in people living with HIV/AIDS | pt_BR |
| Type | Article | |
| DOI | 10.1007/s00430-019-00637-2 | |
| Abstract | Human gammaherpesvirus 8 (HHV-8) replication is influenced by a complex interaction between viral and host elements. Here, we evaluated the expression of NFκB and TNF-α in B (CD19 +) and T (CD3 +) lymphocytes, and the serum concentration of IL-1β and IL-12 cytokines in people living with HIV/AIDS (PLHA), negative for HHV-8-related diseases, and who presented antibodies to latent or lytic antigens from HHV-8. In addition, we also evaluated the correlation of HHV-8 viral load with NFκB, TNF-α, IL-1β and IL-12 levels. The expression of NFκB (p < 0.0001) or TNF-α (p < 0.0001) in B lymphocytes (CD19 +) and the IL-1β (p < 0.0266) and IL-12 (p < 0.0001) concentrations were associated with the presence of antibodies to HHV-8 lytic antigens. The CD19 + NFκB + TNF-α + and CD3 + NFκB + TNF-α + cells were also associated with the presence of antibodies to lytic infection (p < 0.0001). Among all PLHA evaluated, only individuals with the highest titers of lytic antibodies, i.e., 1:320, had detectable HHV-8 viral load. In these, HHV-8 viral load was correlated to NFκB (r = 0.6, p = 0.003) and TNF-α (r = 0.5, p = 0.01) (both in CD19 + lymphocytes) and with IL-1β (r = 0.5, p = 0.01) and IL-12 (r = 0.6, p = 0.006) levels. We believe that viral replication and/or reactivation, in addition to being associated with the development of lytic antibodies against HHV-8, may be associated with inflammatory response via NFκB. Finally, although immune response imbalance has been previously related to HHV-8-associated diseases, our results indicate that important changes in immunity, mainly in the inflammatory response, may be clearly observed in individuals with HHV-8, but who have not yet presented clinical manifestations. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo Asami. Setor de Virologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo Asami. Setor de Virologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo Asami. Setor de Virologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Santa Maria. Departamento de Medicina Veterinária Preventiva. Setor de Virologia. Santa Maria, RS, Brasil / Universidade Federal de Santa Maria. Departamento de Microbiologia e Parasitologia. Santa Maria, RS, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo. Laboratório de Virologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Odontologia. Departamento de Estomatologia. Divisão de Patologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo Asami. Setor de Virologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Fisiologia e Farmacologia. Recife, PE, Brasil. | pt_BR |
| Subject | Antibodies | pt_BR |
| Subject | HHV-8 | pt_BR |
| Subject | IL-12 | pt_BR |
| Subject | IL-1β | pt_BR |
| Subject | Inflammation | pt_BR |
| Subject | NFκB | pt_BR |
| Subject | TNF-α | pt_BR |
| Subject | Viral load | pt_BR |
| DeCS | Anticorpos | pt_BR |
| DeCS | Interleucina-12 | pt_BR |
| DeCS | Inflamação | pt_BR |
| DeCS | Herpesvirus Humano 8 | pt_BR |
| DeCS | Carga Viral | pt_BR |
| Embargo date | 2050-01-01 | |
