| Author | Rolando, Roberta Flávia Ribeiro | |
| Author | Silva, Sidnei da | |
| Author | Peralta, Regina Helena Saramago | |
| Author | Silva, Alexandre Januário da | |
| Author | Cunha, Flavia de Souza | |
| Author | Bello, Alexandre Ribeiro | |
| Author | Peralta, José Mauro | |
| Access date | 2019-10-22T12:07:41Z | |
| Available date | 2019-10-22T12:07:41Z | |
| Document date | 2012 | |
| Citation | ROLANDO, Roberta Flávia Ribeiro et al. Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 4, p. 476-479, June 2012. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/36618 | |
| Language | eng | pt_BR |
| Publisher | Instituto Oswaldo Cruz, Ministério da Saúde | pt_BR |
| Rights | open access | pt_BR |
| Title | Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/S0074-02762012000400006 | |
| Abstract | This study reports the first genetic characterisation of Cryptosporidium isolates in Brazil using real-time polymerase chain reaction (RT-PCR). A total of 1,197 faecal specimens from children and 10 specimens from human immunodeficiency virus-infected patients were collected between 1999-2010 and screened using microscopy. Forty-eight Cryptosporidium oocyst-positive isolates were identified and analysed using a generic TaqMan assay targeting the 18S rRNA to detect Cryptosporidium species and two other TaqMan assays to identify Cryptosporidium hominis and Cryptosporidium parvum. The 18S rRNA assay detected Cryptosporidium species in all 48 of the stool specimens. The C. parvum TaqMan assay correctly identified five/48 stool samples, while 37/48 stool specimens were correctly amplified in the C. hominis TaqMan assay. The results obtained in this study support previous findings showing that C. hominis infections are more prevalent than C. parvum infections in Brazil and they demonstrate that the TaqMan RT-PCR procedure is a simple, fast and valuable tool for the detection and differentiation of Cryptosporidium species. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Centers for Disease Control and Prevention. Center for Global Health. Division of Parasitic Diseases and Malaria. Atlanta, GA, USA | pt_BR |
| Affilliation | Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Cryptosporidium | pt_BR |
| Subject | RT-PCR | pt_BR |
| Subject | Molecular epidemiology | pt_BR |
| e-ISSN | 1678-8060 | |
