| Author | Spano, Liliana Cruz | |
| Author | Ferreira, Mônica Simões Rocha | |
| Author | Almeida, Marilda Santos | |
| Author | Nascimento, Jussara Pereira do | |
| Author | Leite, José Paulo Gagliardi | |
| Access date | 2019-10-22T13:16:50Z | |
| Available date | 2019-10-22T13:16:50Z | |
| Document date | 2007 | |
| Citation | SPANO, Liliana Cruz et al. HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil. Brazilian Journal of Microbiology, São Paulo, v. 38, n. 3, p. 424-429, July/Sept. 2007. | pt_BR |
| ISSN | 1517-8382 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/36628 | |
| Language | eng | pt_BR |
| Publisher | Universidade de São Paulo. Sociedade Brasileira de Microbiologia | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Citomegalovírus | pt_BR |
| Subject in Portuguese | Secreção cervical | pt_BR |
| Subject in Portuguese | Tecidos da placenta | pt_BR |
| Subject in Portuguese | gB Genótipos | pt_BR |
| Subject in Portuguese | Polimorfismo do comprimento do fragmento de restrição | pt_BR |
| Title | HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil | pt_BR |
| Alternative title | Genótipos gB de citomegalovírus humano em secreção cervical e placenta no Espírito Santo, Sudeste do Brasil | pt_BR |
| Type | Article | |
| DOI | 10.1590/S1517-83822007000300008 | |
| Abstract | Human cytomegalovirus (HCMV) displays genetic variability in several regions, supposed to be related with
strain-specific tissue tropism and immunopathogenesis. Based on sequence variation in the UL55 gene that
encodes gB glycoprotein, HCMV strains can be assigned to one of four genotypes. Previous studies have
addressed gB genotyping mostly by investigating strains derived from immunosuppressed patients, sometimes
without previous knowledge about genotype distribution in a geographic area. The present study verified
the distribution of HCMV gB genotypes of strains obtained from immunocompetent women at Vitória City,
Espírito Santo State, Southeastern, Brazil. The HCMV genome was extracted from their cervical secretion,
fetal and maternal placenta tissues (chorionic villous and decidua) from abortion cases and from white blood
cells (WBCs). HCMV genotyping was performed by restriction fragment length polymorphism analyses of
amplified product from the high variability site of the UL55 gene. All four genotypes were observed in both
cervical secretion and placenta, whereas in WBCs a single gB1 genotype was detected. HCMV gB1 and gB2
genotypes were detected, respectively, in nine and in six of the 23 studied samples, while gB3 and gB4 were
each found in four separate samples of the total. The differences in genotype frequency were not considered
statistically significant. No mixed genotype infection was observed. The results indicated that the four gB
HCMV genotypes had no particular tropism for placenta tissues and that all genotypes circulated within
immunocompetent women at the time and in the region of study. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Centro de Ciências da Saúde. Departamento de Patologia e Núcleo de Doenças Infecciosas. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Centro Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Cytomegalovirus | pt_BR |
| Subject | RFLP | pt_BR |
| Subject | gB genotypes | pt_BR |
| Subject | Cervical secretion | pt_BR |
| Subject | Placenta tissues | pt_BR |
| e-ISSN | 1678-4405 | |
