| Author | Silva, Cláudia Maria Dornelles da | |
| Author | Costi, Cintia | |
| Author | Krug, Luciano Percival | |
| Author | Ramos, Ana Beatris | |
| Author | Grandi, Tarciana | |
| Author | Gandolfi, Vitório Luiz | |
| Author | Menezes, Maria Elizabeth | |
| Author | Ocampos, Maristela | |
| Author | Niel, Christian | |
| Author | Rossetti, Maria Lucia Rosa | |
| Access date | 2019-10-26T17:51:05Z | |
| Available date | 2019-10-26T17:51:05Z | |
| Document date | 2007 | |
| Citation | SILVA, Cláudia Maria Dornelles da et al. High proportion of hepatitis C virus genotypes 1 and 3 in a large cohort of patients from Southern Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 7, p. 867-870, Nov. 2007. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/36721 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Vírus da Hepatite C | pt_BR |
| Subject in Portuguese | Genótipos | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Subject in Portuguese | Polimorfismo do comprimento do fragmento de restrição | pt_BR |
| Subject in Portuguese | Sequenciamento de nucleotídeos | pt_BR |
| Subject in Portuguese | Rio Grande do Sul | pt_BR |
| Subject in Portuguese | Santa Catarina | pt_BR |
| Title | High proportion of hepatitis C virus genotypes 1 and 3 in a large cohort of patients from Southern Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1590/s0074-02762007005000122 | |
| Abstract | Hepatitis C virus (HCV) isolates have been divided into six genotypes (1 to 6). The duration of hepatitis C standard treatment is 48 weeks for patients infected with HCV genotype 1 vs 24 weeks for those infected with genotypes 2 and 3. A total of 1544 HCV isolates from chronic patients living in the southern Brazilian states of Rio Grande do Sul (RS, n=627) and Santa Catarina (SC, n=917) were genotyped by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products. In RS, 338 (53.9%; 95% CI 50.0-57.8%), 34 (5.4%; 95% CI 3.8-7.4%) and, 255 (40.7%; 95% CI 36.9-44.6%) samples were from genotypes 1, 2, and 3, respectively. In SC, 468 (51%; 95% CI 47.8-54.2%), 26 (2.9%; 95% CI 1.9-4.1%) and, 423 (46.1%; 95% CI 42.9-49.3%) samples were from genotypes 1, 2, and 3, respectively. Genotyping results were confirmed by direct nucleotide sequencing of PCR products derived from 68 samples, without any discrepancy between PCR-RFLP and nucleotide sequencing methods. In conclusion, almost half of the hepatitis C patients from South of Brazil are infected by genotypes 2 and 3 and, these results have important consequential therapeutic implications as they can be treated for only 24 weeks, not 48. | pt_BR |
| Affilliation | Secretaria de Saúde do Estado do Rio Grande do Sul. Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico do Estado do Rio Grande do Sul. Porto Alegre, RS, Brasil / Universidade Luterana do Brasil. Canoas, RS, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Laboratório Amplicon. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Fundação de Cardiologia. Alvorada, RS, Brasil. | pt_BR |
| Affilliation | Secretaria de Saúde do Estado do Rio Grande do Sul. Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico do Estado do Rio Grande do Sul. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Secretaria Estadual de Saúde do Estado de Santa Catarina. Laboratório Central de Santa Catarina. Setor de Virologia. Florianópolis, SC, Brasil. | pt_BR |
| Affilliation | Laboratório DNAnalise; Florianópolis, SC, Brasil. | pt_BR |
| Affilliation | Instituto de Biotecnologia Aplicada. Florianópolis, SC, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Luterana do Brasil. Canoas, RS, Brasil. | pt_BR |
| Subject | Hepatitis C virus genotypes | pt_BR |
| Subject | Polymerase chain reaction | pt_BR |
| Subject | Restriction fragment lengh polymorphism | pt_BR |
| Subject | Nucleotide sequencing | pt_BR |
| Subject | Rio Grande do Sul | pt_BR |
| Subject | Santa Catarina | pt_BR |
| e-ISSN | 1678-8060 | |
