| Author | Santos, Marcia R. M. | |
| Author | Cano, Maria I. | |
| Author | Schijman, Alejandro | |
| Author | Lorenzi, Hernan | |
| Author | Vázquez, Martin | |
| Author | Levin, Mariano J. | |
| Author | Ramirez, José Luis | |
| Author | Brandão, Adeilton | |
| Author | Degrave, Wim M. | |
| Author | Silveira, José Franco da | |
| Access date | 2019-11-02T19:50:35Z | |
| Available date | 2019-11-02T19:50:35Z | |
| Document date | 1997 | |
| Citation | SANTOS, Marcia R. M. et al. The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 8, p. 821-828, Nov./Dec. 1997. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/36813 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Cone CL Brener | pt_BR |
| Subject in Portuguese | Projeto Genoma | pt_BR |
| Subject in Portuguese | Mapa cromossômico | pt_BR |
| Subject in Portuguese | Mapa genético | pt_BR |
| Subject in Portuguese | Cariótipo molecular | pt_BR |
| Title | The Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brener | pt_BR |
| Type | Article | |
| DOI | 10.1590/s0074-02761997000600018 | |
| Abstract | By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, INGEBI-CONICET, FCEyN-UBA. Buenos Aires, Argentina. | pt_BR |
| Affilliation | Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, INGEBI-CONICET, FCEyN-UBA. Buenos Aires, Argentina. | pt_BR |
| Affilliation | Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, INGEBI-CONICET, FCEyN-UBA. Buenos Aires, Argentina. | pt_BR |
| Affilliation | Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, INGEBI-CONICET, FCEyN-UBA. Buenos Aires, Argentina. | pt_BR |
| Affilliation | Universidad Central de Venezuela. Caracas, Venezuela. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. São Paulo, SP, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Cone CL Brener | pt_BR |
| Subject | Genome project | pt_BR |
| Subject | Molecular karyotype | pt_BR |
| Subject | Chromosome mapping | pt_BR |
| Subject | Genetic map | pt_BR |
| e-ISSN | 1678-8060 | |
