| Author | Degrave, Wim M. | |
| Author | Fernandes, Octavio | |
| Author | Campbell, David | |
| Author | Bozza, Marcelo | |
| Author | Lopes, Ulisses | |
| Access date | 2019-11-03T15:00:25Z | |
| Available date | 2019-11-03T15:00:25Z | |
| Document date | 1994 | |
| Citation | DEGRAVE, Wim et al. Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 89, n. 3, p. 463-469, July/Sept. 1994. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/36819 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| MeSH | DNA de Cinetoplasto | pt_BR |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Subject in Portuguese | Hibridização | pt_BR |
| Subject in Portuguese | Sondas Moleculares | pt_BR |
| Subject in Portuguese | DNA de Cinetoplasto | pt_BR |
| Title | Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02761994000300032 | |
| Abstract | The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of California. School of Medicine. Department of Microbiology and Immunology. Los Angeles, USA. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Leishmania | pt_BR |
| Subject | Polymerase chain reaction | pt_BR |
| Subject | Hybridization | pt_BR |
| Subject | Molecular probes | pt_BR |
| Subject | Mini-exon gene | pt_BR |
| Subject | Minicircle | pt_BR |
| Subject | kDNA | pt_BR |
| e-ISSN | 1678-8060 | |
