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| Autor | Luz, Mauricio R. M. P. | |
| Autor | Van Leuven, Fred | |
| Autor | Araujo Jorge, Tania C. | |
| Fecha de acceso | 2019-11-05T12:08:20Z | |
| Fecha de disponibilización | 2019-11-05T12:08:20Z | |
| Fecha de publicación | 1994 | |
| Referencia | LUZ, Mauricio R. M. P.; VAN LEUVEN, Fred; ARAUJO JORGE, Tania C. An inhibition ELISA to determine α macroglobulin levels in mouse plasma. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 89, n. 2, p. 203-207, Apr./June 1994. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/36854 | |
| Idioma | eng | pt_BR |
| Editor | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Derechos de autor | open access | |
| Palabras clave en Portugués | Murinoglobulina | pt_BR |
| Palabras clave en Portugués | ELISA | pt_BR |
| Título | An inhibition ELISA to determine alpha macroglobulin levels in mouse plasma | pt_BR |
| Tipo del documento | Article | |
| DOI | 10.1590/s0074-02761994000200014 | |
| Resumen en Inglés | A sensitive method for quantifying mouse plasma alpha-macroglobulins (AM) using an inhibition ELISA is described. AM are important plasma proteinase inhibitors that possibly act also as immunomodulatory molecules. The standard protocol developed in our experiments involves coating well with 10 micrograms/ml A2M in carbonate buffer, followed by incubation with a 1:1 (v/v) mixture of the plasma to be tested (diluted 1/1000) and goat anti-AM (diluted 1/1250). This is followed by further incubation, first with the enzyme-conjugated antibody and with the substrate prior to the reading of absorbance levels of the reaction products. Standard curve samples must be included in each plate, employing known amounts of the purified Murine Alpha-2-Macroglobulin (MuA2M) used for coating, with concentrations ranging from 0.001 to 10 micrograms/ml. Using test samples in triplicates and a 6-point standard curve in a single ELISA plate, 25 plasma samples can be tested accurately. The method offers an useful tool for establishing AM levels in small samples of mouse plasma. | pt_BR |
| Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliación | Katholic University of Leuven. Dept. of Human Genetics. Leuven, Belgium. | pt_BR |
| Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Palavras clave en Inglês | α-2-macroglobulin | pt_BR |
| Palavras clave en Inglês | A2M | pt_BR |
| Palavras clave en Inglês | ELISA | pt_BR |
| Palavras clave en Inglês | Murinoglobulin | pt_BR |
| e-ISSN | 1678-8060 |
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