| Author | Pinto, Patricia Machado | |
| Author | Brito, Cristiana F. A. | |
| Author | Passos, Liana K. Jannoti | |
| Author | Tendler, Miriam | |
| Author | Simpson, Andrew J. G. | |
| Access date | 2019-11-05T13:43:05Z | |
| Available date | 2019-11-05T13:43:05Z | |
| Document date | 1997 | |
| Citation | PINTO, Patricia Machado et al. Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 3, p. 409-414, May/June 1997. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/36863 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Schistosoma mansoni | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Subject in Portuguese | DNA polimórfico amplificado aleatoriamente | pt_BR |
| Subject in Portuguese | Esquistossomose | pt_BR |
| Title | Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni | pt_BR |
| Type | Article | |
| DOI | 10.1590/s0074-02761997000300019 | |
| Abstract | The extent of genomic variability of clones of Schistosoma mansoni obtained from field isolates was compared with that of strains that have been laboratory maintained. Analysis was undertaken using randomly amplified polymorphic DNAs (RAPDs) generated with three primers. Phenograms showing the similarity among the clones were constructed. The data showed that while the laboratory strain is highly homogeneous the clones derived from the field populations were highly variable with 43% of RAPDs exhibiting polymorphisms among 23 clones. Clones isolated from the same infected individual were always more closely grouped than clones from different individuals. The data clearly demonstrated that earlier analyses of the genomic variability in S. mansoni have underestimated this phenomenon due to the failure to examine field isolates. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte. MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malacologia. Belo Horizonte. MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malacologia. Belo Horizonte. MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Ludwig de Pesquisas sobre o Câncer. Laboratório de Genética de Câncer. São Paulo, SP, Brasil. | pt_BR |
| Subject | Polymerase chain reaction | pt_BR |
| Subject | Randomly amplified polymorphic DNA | pt_BR |
| Subject | Schistosomiasis | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| e-ISSN | 1678-9849 | |
