Author | Díaz-Varela, Míriam | |
Author | Menezes-Neto, Armando de | |
Author | Perez-Zsolt, Daniel | |
Author | Gámez-Valero, Ana | |
Author | Seguí-Barber, Joan | |
Author | Izquierdo-Useros, Nuria | |
Author | Martinez-Picado, Javier | |
Author | Fernández-Becerra, Carmen | |
Author | Portillo, Hernando A. del | |
Access date | 2019-11-06T15:09:17Z | |
Available date | 2019-11-06T15:09:17Z | |
Document date | 2018 | |
Citation | DÍAZ-VARELA, M. et al. Proteomics study of human cord blood reticulocyte-derived exosomes. Scientific Reports, v. 8, n. 1, p. 1-11, 2018. | pt_BR |
ISSN | 2045-2322 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/36904 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | Proteomics study of human cord blood reticulocyte-derived exosomes | pt_BR |
Type | Article | pt_BR |
DOI | 10.1038/s41598-018-32386-2 | |
Abstract | Reticulocyte-derived exosomes (Rex), extracellular vesicles of endocytic origin, were initially discovered as a cargo-disposal mechanism of obsolete proteins in the maturation of reticulocytes into erythrocytes. In this work, we present the first mass spectrometry-based proteomics of human Rex (HuRex). HuRex were isolated from cultures of human reticulocyte-enriched cord blood using different culture conditions and exosome isolation methods. The newly described proteome consists of 367 proteins, most of them related to exosomes as revealed by gene ontology over-representation analysis and include multiple transporters as well as proteins involved in exosome biogenesis and erythrocytic disorders. Immunoelectron microscopy validated the presence of the transferrin receptor. Moreover, functional assays demonstrated active capture of HuRex by mature dendritic cells. As only seven proteins have been previously associated with HuRex, this resource will facilitate studies on the role of human reticulocyte-derived exosomes in normal and pathological conditions affecting erythropoiesis. | pt_BR |
Affilliation | Universidade de Barcelona. Hospital Clínic. ISGlobal. Barcelona, Espanha. | pt_BR |
Affilliation | Universidade de Barcelona. Hospital Clínic. ISGlobal. Barcelona, Espanha / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Instituto de Pesquisa em AIDS IrsiCaixa. Badalona, Espanha. | pt_BR |
Affilliation | Universidade de Barcelona. Hospital Clínic. ISGlobal. Barcelona, Espanha / Universitat Autònoma de Barcelona. Hospital Universitário e Instituto de Pesquisa em Ciências da Saúde Germans Trias i Pujol. Departamento de Patologia e Grupo REMAR-IVECAT. Barcelona, Espanha. | pt_BR |
Affilliation | Universidade de Barcelona. Hospital Clínic. ISGlobal. Barcelona, Espanha. | pt_BR |
Affilliation | Instituto de Pesquisa em AIDS IrsiCaixa. Badalona, Espanha / Instituto IGTP de Investigação Alemão Trias i Pujol. Badalona, Espanha. | pt_BR |
Affilliation | Instituto de Pesquisa em AIDS IrsiCaixa. Badalona, Espanha / Universitat de Vic - Universidade Central da Catalunha. Vic, Espanha / Instituto Catalão de Estudos e Estudos Avançados. Barcelona, Espanha. | pt_BR |
Affilliation | ISGlobal, Hospital Clínic - Universidade de Barcelona. Barcelona, Espanha / Instituto IGTP de Investigação Alemão Trias i Pujol. Badalona, Espanha. | pt_BR |
Affilliation | ISGlobal, Hospital Clínic - Universidade de Barcelona. Barcelona, Espanha / Instituto IGTP de Investigação Alemão Trias i Pujol. Badalona, Espanha / Instituto Catalão de Estudos e Estudos Avançados. Barcelona, Espanha. | pt_BR |
Subject | Proteomics | pt_BR |
Subject | Human cord blood | pt_BR |
Subject | Reticulocyte-derived exosomes | pt_BR |
DeCS | Bancos de Sangue | pt_BR |
DeCS | Técnicas de Cultura de Células | pt_BR |
DeCS | Células Cultivadas | pt_BR |
DeCS | Células Dendríticas / citologia | pt_BR |
DeCS | Células Dendríticas / metabolismo | pt_BR |
DeCS | Exossomos / metabolismo | pt_BR |
DeCS | Sangue Fetal / citologia | pt_BR |
DeCS | Sangue Fetal / metabolismo | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Espectrometria de Massas | pt_BR |
DeCS | Microscopia Imunoeletrônica | pt_BR |
DeCS | Nanotecnologia | pt_BR |
DeCS | Proteômica / métodos | pt_BR |
DeCS | Receptores da Transferrina / metabolismo | pt_BR |
DeCS | Reticulócitos / citologia | pt_BR |
DeCS | Reticulócitos / metabolismo | pt_BR |