| Author | Quintaes, Bianca R. | |
| Author | Leal, Nilma C. | |
| Author | Reis, Eliane M. F. | |
| Author | Fonseca, Erica L. | |
| Author | Hofer, Ernesto | |
| Access date | 2019-11-14T11:26:03Z | |
| Available date | 2019-11-14T11:26:03Z | |
| Document date | 2002 | |
| Citation | QUINTAES, Bianca R. et al. Conventional and molecular typing of Salmonella Typhi strains from Brazil. Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 44, n. 6, p. 315-319, Nov./Dec. 2002. | pt_BR |
| ISSN | 0036-4665 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/37043 | |
| Language | eng | pt_BR |
| Publisher | Instituto de Medicina Tropical de São Paulo | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Salmonella Typhi | pt_BR |
| Subject in Portuguese | Tipagem | pt_BR |
| Subject in Portuguese | Reação em cadeia polimórfica aleatória amplificada em DNA-polimerase | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Title | Conventional and molecular typing of Salmonella typhi strains from Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1590/s0036-46652002000600004 | |
| Abstract | Phenotypic and genotypic characteristics of Salmonella Typhi were studied in 30 strains, isolated in different years, from some areas in Brazil. Conventional typing methods were performed by biochemical tests, Vi phage-typing scheme, and antimicrobial susceptibility test. Molecular typing methods were performed by analysis of plasmid DNA and by random amplified polymorphic DNA (RAPD-PCR). For the latter, an optimization step was performed to ensure the reproducibility of the process in genetic characterization of S. Typhi. The predominance of 76.7% of biotype I (xylose +, arabinose -) was noticed in all studied areas. Three phage types were recognized, with prominence for the phage types A (73.3%) and I+IV (23.3%). All the strains were susceptible to the drugs used. However, 36.7% of the strains contained plasmids, with predominance of the 105 Kb plasmid. RAPD was capable of grouping the strains in 8 genotypic patterns using primer 784, in 6, using primer 787 and in 7, using primer 797. Conventional phenotypic typing methods, as well as the DNA plasmid analysis, presented nonsignificant discriminatory power; however, RAPD-PCR analysis showed discriminatory power, reproducibility, easy interpretation and performance, being considered as a promising alternative typing method for S. Typhi. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia Médica, Curso de Pós-Graduação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Typing | pt_BR |
| Subject | Salmonella Typhi | pt_BR |
| Subject | Vi Phage-typing | pt_BR |
| Subject | Random Amplified Polymorphic DNA-Polymerase Chain Reaction | pt_BR |
| Subject | Brazil | pt_BR |
| e-ISSN | 1678-9946 | |
