| Author | Tomazett, Patrícia Kott | |
| Author | Castro, Nadya da Silva | |
| Author | Lenzi, Henrique Leonel | |
| Author | Soares, Célia Maria de Almeida | |
| Author | Pereira, Maristela | |
| Access date | 2011-12-13T13:31:57Z | |
| Available date | 2011-12-13T13:31:57Z | |
| Document date | 2011 | |
| Citation | TOMAZETT, Patrícia Kott et al. Response of Paracoccidioides brasiliensis Pb01 to stressor agents and cell wall osmoregulators. Fungal Biology, v. 115, p. 62-69, Jan. 2011. | pt_BR |
| ISSN | 1878-6146 | |
| URI | https://www.arca.fiocruz.br/handle/icict/3707 | |
| Description | This study at Universidade Federal de Goiás was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Financiadora de Estudos e Projetos (FINEP) and by the International Foundation for Sciences (IFS), Stockholm, Sweden, through a grant to M.P. P.K.T. was supported by a fellowship from CNPq. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | en |
| Rights | open access | |
| Subject in Portuguese | Fungo Dimórfico | pt_BR |
| Subject in Portuguese | Paracoccidioides brasiliensis | pt_BR |
| Subject in Portuguese | Glucosamina | pt_BR |
| Subject in Portuguese | Síntese Química | pt_BR |
| Title | Response of Paracoccidioides brasiliensis Pb01 to stressor agents and cell wall osmoregulators | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.funbio.2010.10.005 | |
| Abstract | In its attempt to survive, the fungal cell can change the cell wall composition and/or structure in response to environmental stress. The molecules involved in these compensatory mechanisms are a possible target for the development of effective antifungal agents. In the thermodimorphic fungus Paracoccidioides brasiliensis Pb01, the main polymers that compose the cell wall are chitin and glucans. These polymers form a primary barrier that is responsible for the structural integrity and formation of the cell wall. In this study the behaviour of P. brasiliensis was evaluated under incubation with cell wall stressor agents such as Calcofluor White (CFW), Congo Red (CR), Sodium Dodecyl Sulphate (SDS), NaCl, KCl, and Sorbitol. Use of concentrations at which the fungus is visually sensitive to those agents helped to explain some of the adaptive mechanisms used by P. brasiliensis in response to cell wall stress. Our results show that 1,3-β-D-glucan synthase (PbFKS1), glucosamine-6-phosphate synthase (PbGFA1) and β-1,3-glucanosyltransferase (PbGEL3)as well as 1,3-β-D-glucan and N-acetylglucosamine (GlcNAc) residues in the cell wall are involved in compensatory mechanisms against cell wall damage. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Departamento de Bioquímica e Biologia Molecular. Goiás, GO, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Departamento de Bioquímica e Biologia Molecular. Goiás, GO, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Departamento de Bioquímica e Biologia Molecular. Goiás, GO, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Departamento de Bioquímica e Biologia Molecular. Goiás, GO, Brasil. | pt_BR |
| Subject | Cell Wall Integrity | pt_BR |
| Subject | Glucanosyltransferase | pt_BR |
| Subject | Glucosamine-6-phosphate synthase | pt_BR |
| Subject | Paracoccidoides brasiliensis | pt_BR |
| Subject | Stress Conditions | pt_BR |
| DeCS | Fungos | pt_BR |
| DeCS | Paracoccidioides | pt_BR |
| DeCS | Glucosamina | pt_BR |
| DeCS | Síntese Química | pt_BR |
