| Author | Lopera, Damaris | |
| Author | Naranjo, Tonny W. | |
| Author | Cruz, Oswaldo Gonçalves | |
| Author | Restrepo, Angela | |
| Author | Cano, Elena | |
| Author | Lenzi, Henrique Leonel | |
| Access date | 2011-12-13T16:38:11Z | |
| Available date | 2011-12-13T16:38:11Z | |
| Document date | 2011 | |
| Citation | LOPERA, Damaris et al. Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice. Plos Neglected Tropical Diseases, v. 5, p. 1-9, 2011. | pt_BR |
| ISSN | 1935-2727 | |
| URI | https://www.arca.fiocruz.br/handle/icict/3709 | |
| Description | Conceived and designed the experiments: LEC HLL DL TWN. Performed the experiments: DL TWN. Analyzed the data: DL HLL OGC LEC. Contributed reagents/materials/analysis tools: HLL. Wrote the paper: DL HLL AR. Scientific advisor of the work who reviewed the manuscript: AR LEC. | pt_BR |
| Language | eng | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Paracoccidioidomicose | pt_BR |
| Subject in Portuguese | Doenças Endêmicas | pt_BR |
| Subject in Portuguese | Paracoccidioides | pt_BR |
| Title | Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pntd.0001232 | |
| Abstract | Background: Paracoccidioidomycosis (PCM), an endemic systemic mycosis caused by the fungus Paracoccidioides brasiliensis (Pb), usually results in severe lung damage in patients. Methods and Findings: Considering the difficulties to sequentially study the infection in humans, this work was done in mice inoculated intranasally with infective Pb-conidia. Lungs of control and Pb-infected mice were studied after 2-hours, 4, 8, 12 and 16-weeks post-infection (p.i) in order to define histopathologic patterns of pulmonary lesions, multiplex-cytokine profiles and their dynamics during the course of this mycosis. Besides the nodular/granulomatous lesions previously informed, results revealed additional non-formerly described lung abnormalities, such as periarterial sheath inflammation and pseudotumoral masses. The following chronologic stages occurring during the course of the experimental infection were defined: Stage one (2-hours p.i): mild septal infiltration composed by neutrophils and macrophages accompanied by
an intense ‘‘cytokine burst’’ represented by significant increases in IL-1a, IL-1b, IL-4, IL-5, IL-6, IL-10, IL12p70, IL-13, IL-17, Eotaxin, G-CSF, MCP1, MIP1a, GM-CSF, IFN-c, MIP1b and TNFa levels. Stage two (4-weeks p.i): presence of nodules, evidence of incipient periarterial- and intense but disperse parenchymal- inflammation, abnormalities that continued to be
accompanied by hyper-secretion of those cytokines and chemokines mentioned in the first stage of infection. Stages three and four (8 and 12-weeks p.i.): fungal proliferation, inflammation and collagenesis reached their highest intensity with particular involvement of the periarterial space. Paradoxically, lung cytokines and chemokines were down-regulated with
significant decreases in IL-2,IL-3,IL-5,IL-9,IL-13,IL-15,GM-CSF,IFN-c,MIP1b and TNFa. Stage five (16-weeks p.i.): inflammation decreased becoming limited to the pseudotumoral masses and was accompanied by a ‘‘silent’’ cytokine response, except for PDGF, MIG, RANTES and IL12p40 which remained up-regulated for the duration of the experiment. Conclusions: Results of this study identified both classic and novel patterns corresponding to histopathologic and immunologic responses occurring during the course of experimental PCM. | pt_BR |
| Affilliation | Corporación para Investigaciones Biológicas. Medical and Experimental Mycology Group. Medellín, Colômbia. | pt_BR |
| Affilliation | Corporación para Investigaciones Biológicas. Medical and Experimental Mycology Group, Medellín, Colômbia / Universidad Pontificia Bolivarina. School of Health Sciences. Medellín, Colômbia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Presidência. Programa de Computação Científica.Rio de Janeiro, Brasil. | pt_BR |
| Affilliation | Corporación para Investigaciones Biológicas. Medical and Experimental Mycology Group. Medellín, Colômbia. | pt_BR |
| Affilliation | Corporación para Investigaciones Biológicas. Medical and Experimental Mycology Group. Medellín, Colômbia / Universidad Pontificia Bolivarina. School of Health Sciences. Medellín, Colômbia / Universidad de Antioquia. Microbiology School. Medellín, Colômbia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Paracoccidioidomycosis | pt_BR |
| Subject | Endemic Systemic Mycosis | pt_BR |
| Subject | Paracoccidioides brasiliensis | pt_BR |
| DeCS | Paracoccidioidomicose | pt_BR |
| DeCS | Doenças Endêmicas | pt_BR |
| DeCS | Paracoccidioides | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
