| Author | Ribeiro, Flávia Andressa Pidone | |
| Author | Pontes, Camila | |
| Author | Machado, Alexandre de M.V. | |
| Author | Bruna‐Romero, Oscar | |
| Author | Quintana, Hananiah T. | |
| Author | Oliveira, Flávia de | |
| Author | Vasconcelos, José Ronnie Carvalho de | |
| Author | Ribeiro, Daniel Araki | |
| Access date | 2019-11-20T15:33:14Z | |
| Available date | 2019-11-20T15:33:14Z | |
| Document date | 2019 | |
| Citation | RIBEIRO, Flávia Andressa Pidone et al. Therapeutical effects of vaccine from Trypanosoma cruzi amastigote surface protein 2 by simultaneous inoculation with live parasites. Journal of Cellular Biochemistry, v. 120, n. 3, p. 3373-3383, 2019. | pt_BR |
| ISSN | 0730-2312 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/37202 | |
| Language | eng | pt_BR |
| Publisher | Wiley Periodicals | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Therapeutical effects of vaccine from Trypanosoma cruzi amastigote surface protein 2 by simultaneous inoculation with live parasites | pt_BR |
| Type | Article | |
| DOI | 10.1002/jcb.27608 | |
| Abstract | The aim of this study was to evaluate the efficacy of vaccine using replication-deficient human recombinant Type 5 replication-defective adenoviruses (AdHu5) carrying sequences of the amastigote surface protein 2 (ASP2) (AdASP2) in mice infected with the Trypanosoma cruzi ( T cruzi) Y strain. A total of 16 A/Sn mice female were distributed into four groups, as follows (n = 4 per group): Group 1 - Control Group (CTRL); Group 2 - Infected Group (TC): animals were infected by subcutaneous route with 150 bloodstream trypomastigotes of T cruzi Y strain; Group 3 - Immunized Group (AdASP-2): animals were immunized by intramuscular injection (im) route with 50 µL of AdSP-2 (2 × 10 8 plaque forming units [pfu]/cam) at day 0; Group 4-Immunized and Infected Group (AdASP-2+TC): animals were immunized by im route with 50 µL of ASP-2 (2 × 10 8 pfu/cam) and infected by T cruzi at the same day (day 0). It was observed a significant decrease of nests in the group that was immunized with AdASP-2 and infected on the same day. Tumor necrosis factor alpha (TNF-α) and inducible nitric oxide synthase (iNOS) gene expressions showed a significant increase in the AdASP-2+TC group when compared to TC group, but it was noted that Cyclooxygenase-2 (Cox-2) was increased in TC group when compared to AdASP-2+TC group. Increase of matrix metalloproteinases-2 (MMP-2) and decrease of MMP-9 immunoexpression in the AdASP-2+TC group was noticed as well. Oxidative DNA damage was present in myocardium for AdASP-2+TC group as a result of 8-hydroxydeoxyguanosine immunoexpression. Taken together, our results highlighted an increased oxidative stress, MMP-2 activity and inflammatory host response promoted by AdASP-2 against T cruzi infection. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Biociências. Santos, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Biociências. Santos, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Biociências. Santos, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Biociências. Santos, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Biociências. Santos, SP, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi (T cruzi) | pt_BR |
| Subject | Amastigote surface protein-2 (ASP-2) | pt_BR |
| Subject | Cardiomyocyte | pt_BR |
| Subject | Gene expression | pt_BR |
| Subject | Histopathology | pt_BR |
| Subject | Vaccine | pt_BR |
| Embargo date | 2035-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
