| Author | Magalhães, Kelly Grace | |
| Author | Almeida, Patricia E. | |
| Author | Atella, Georgia C. | |
| Author | Monteiro, Clarissa M Maya | |
| Author | Faria Neto, Hugo C. Castro | |
| Author | Machado, Marcelo Pelajo | |
| Author | Lenzi, Henrique Leonel | |
| Author | Bozza, Marcelo T. | |
| Author | Bozza, Patrícia T. | |
| Access date | 2012-01-10T16:12:29Z | |
| Available date | 2012-01-10T16:12:29Z | |
| Document date | 2010 | |
| Citation | MAGALHÃES, Kelly G. et al. Schistosomal-Derived Lysophosphatidylcholine are involved in eosinophil activation and recruitment through toll-like receptor-2-dependent mechanisms. The Journal of Infectious Diseases, v. 202, p. 1369-1379, 2010. | pt_BR |
| ISSN | 0022-1899 | |
| URI | https://www.arca.fiocruz.br/handle/icict/3729 | |
| Description | Acknowledgments - We thank the Program for Technological Development in Tools for Health-PDTISFIOCRUZ, for use of its Luminex and confocal laser scanning microscopy (CLSM) facility, and Pedro Paulo Manso, for assistance with CLSM analysis. | pt_BR |
| Sponsorship | Financial support: PAPES-FIOCRUZ, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil); Fundação de Amparo à Pesquisa do Rio de Janeiro (FAPERJ, Brazil). | pt_BR |
| Language | eng | pt_BR |
| Rights | open access | |
| Title | Schistosomal-Derived Lysophosphatidylcholine Are Involved in Eosinophil Activation and Recruitment through Toll-Like Receptor-2-Dependent Mechanisms | pt_BR |
| Type | Article | |
| DOI | 10.1086/656477 | |
| Abstract | Parasite-derived lipids may play important roles in host-pathogen interactions and escape mechanisms. Herein, we evaluated the role of schistosomal-derived lipids in Toll-like receptor (TLR)-2 and eosinophil activation in Schistosoma mansoni infection. Mice lacking TLR2 exhibited reduced liver eosinophilic granuloma, compared with that of wild-type animals, following S. mansoni infection. Decreased eosinophil accumulation and eosinophil lipid body (lipid droplet) formation, at least partially due to reduced production of eotaxin, interleukin (IL)-5, and IL-13 in S. mansoni-infected TLR2_/_ mice, compared with the corresponding production in wildtype mice, was noted. Although no differences were observed in survival rates during the acute schistosomal infection (up to 50 days), increased survival of TLR2_/_ mice, compared with survival of wild-type mice, was observed during the chronic phase of infection. Schistosomal lipid extract- and schistosomal-derived lysophosphatidylcholine (lyso-PC) stimulated macrophages in vitro induced TLR2-dependent NF-kB activation and cytokine production. Furthermore, in vivo schistosomal lyso-PC administration induced eosinophil recruitment and cytokine production, in a mechanism largely dependent on TLR2. Taken together, our results suggest that schistosomal-derived lyso-PC may participate in cytokine production and eosinophil activation through a TLR2-dependent pathway in S. mansoni infection. Moreover, our results suggest thatTLR2-dependent inflammatory reaction, cytokine production, and eosinophil recruitment and activation may contribute to the pathogenesis and lethality in the chronic phase of infection. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasill | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | Lysophosphatidylcholines | pt_BR |
| Subject | Cytokines | pt_BR |
| Subject | Eosinophils | pt_BR |
| Subject | Toll-Like Receptor | pt_BR |
| Subject | Immunology | pt_BR |
| DeCS | Schistosoma mansoni | pt_BR |
| DeCS | Eosinófilos | pt_BR |
| DeCS | Imunologia | pt_BR |
