| Author | Cruz, Maria da Fátima Ferreira | |
| Author | Passo, Roberto Machado | |
| Author | Fortier, Bernardo | |
| Author | Ribeiro, Cláudio Tadeu Daniel | |
| Access date | 2019-11-26T17:25:01Z | |
| Available date | 2019-11-26T17:25:01Z | |
| Document date | 1992 | |
| Citation | CRUZ, Maria de Fátima Ferreira da et al. Development of an immunoenzymatic assay using a monoclonal antibody against a 50-kDa catabolite from the P126 Plasmodium falciparum protein to the diagnosis of malaria infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, Suppl. 3, p. 187-192, 1992. | pt_BR |
| ISSN | 0074-0206 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/37400 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Plasmodium falciparum | pt_BR |
| Subject in Portuguese | Plasmodium vivax | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Subject in Portuguese | Antígeno plamodial | pt_BR |
| Subject in Portuguese | Anticorpos | pt_BR |
| Subject in Portuguese | Ensaio imunoenzimático | pt_BR |
| Title | Development of an immunoenzymatic assay using a monoclonal antibody against a 50-kDa catabolite from the P126 Plasmodium falciparum protein to the diagnosis of malaria infection | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02761992000700031 | |
| Abstract | The WHO criterion of defering any donation of blood by a confirmed case of malaria for three years after cessation of therapy can not be applied in areas where malaria in endemic. For this reason we developed an immunoenzymatic assay for the detection of plasmodial antigens for blood screening in malararial endemic areas. So, we tested sera from 191 individuals. Among patients with active disease 100% of the cases of Plasmodium falciparum or mixed infections and 91.7% of those with P. vivax were positive for the presence of plasmodial antigens. The lower parasitaemia detected was 0.0003% for P. vivax malária. When the frequency of positive circulating malarial antigens was evaluated among asymptomatic and symptomatic individuals with negative TBS, positive results were found in respectively 38.7% and 17.7% of the individuals studied in the 30 days after confirmed malaria attack. Data provide by these assays have shown that ELISA seemed to be more sensitive than parasitological examination for malaria diagnosis. This test by virtue of its high sensivity and the facilities in processing a large number of specimens, can prove to be useful in endemic areas for the recognition of asymptomatic malaria and screening of blood donors. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro Colaborador da WHO para Pesquisa e Treinamento em Doenças Parasitárias. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro Colaborador da WHO para Pesquisa e Treinamento em Doenças Parasitárias. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Unite Inserum U 42. Villeneuve d'Aseq. France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro Colaborador da WHO para Pesquisa e Treinamento em Doenças Parasitárias. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Plasmodium falciparum | pt_BR |
| Subject | Plasmodium vivax | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Plamodial antigen | pt_BR |
| Subject | Monocional | pt_BR |
| Subject | Antibody | pt_BR |
| Subject | Immunoenzymatic assay | pt_BR |
| e-ISSN | 1678-8060 | |
