| Author | Carvalho, Leonardo José de Moura | |
| Author | Cruz, Maria de Fátima Ferreira da | |
| Author | Daniel-Ribeiro, Cláudio Tadeu | |
| Author | Machado, Marcelo Pelajo | |
| Author | Lenzi, Henrique Leonel | |
| Access date | 2012-01-19T15:04:24Z | |
| Available date | 2012-01-19T15:04:24Z | |
| Document date | 2007 | |
| Citation | CARVALHO, L. J. et al. Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice. Malaria Journal, v.16, n.6, p.59, May 2007. | pt_BR |
| ISSN | 475-2875 | |
| URI | https://www.arca.fiocruz.br/handle/icict/3741 | |
| Description | Acknowledgements to Belmira Santos from the Fiocruz Animal House for providing the CBA mice, and Bruno Eschenazi from the Laboratory of Production and Treatment of Images/IOC/Fiocruz for processing the images. This work was supported with funds of the Laboratory of Malaria Research and the Department of Pathology (POM). | pt_BR |
| Language | eng | pt_BR |
| Publisher | BioMed Central | en |
| Rights | open access | |
| Title | Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice | pt_BR |
| Type | Article | |
| DOI | 10.1186/1475-2875-6-59 | |
| Abstract | Background
Immune responses to malaria blood stage infection are in general defective, with the need for long-term exposure to the parasite to achieve immunity, and with the development of immunopathology states such as cerebral malaria in many cases. One of the potential reasons for the difficulty in developing protective immunity is the poor development of memory responses. In this paper, the potential association of cellular reactivity in lymphoid organs (spleen, lymph nodes and Peyer's patches) with immunity and pathology was evaluated during Plasmodium berghei ANKA infection in CBA mice.
Methods
CBA mice were infected with 1 × 106 P. berghei ANKA-parasitized erythrocytes and killed on days 3, 6–8 and 10 of infection. The spleen, lymph nodes and Peyer's patches were collected, fixed in Carson's formalin, cut in 5 μm sections, mounted in glass slides, stained with Lennert's Giemsa and haematoxylin-eosin and analysed with bright-field microscopy.
Results
Early (day 3) strong activation of T cells in secondary lymphoid organs was observed and, on days 6–8 of infection, there was overwhelming activation of B cells, with loss of conventional germinal center architecture, intense centroblast activation, proliferation and apoptosis but little differentiation to centrocytes. In the spleen, the marginal zone disappeared and the limits between the disorganized germinal center and the red pulp were blurred. Intense plasmacytogenesis was observed in the T cell zone.
Conclusion
The observed alterations, especially the germinal center architecture disturbance (GCAD) with poor centrocyte differentiation, suggest that B cell responses during P. berghei ANKA infection in mice are defective, with potential impact on B cell memory responses. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Mice | pt_BR |
| Subject | Germinal Center | pt_BR |
| Subject | B-Lymphocytes | pt_BR |
| Subject | Immunology | pt_BR |
| Subject | Plasmodium berghei | pt_BR |
| Subject | T-Lymphocytes | pt_BR |
| Subject | Malaria | pt_BR |
| DeCS | Malária | pt_BR |
| DeCS | Imunologia | pt_BR |
| DeCS | Plasmodium berghei | pt_BR |
| DeCS | Centro Germinativo | pt_BR |
| DeCS | Linfócitos B | pt_BR |
| DeCS | Linfócitos T | pt_BR |
| DeCS | Camundongos | pt_BR |
