| Author | Lavich, T. R. | |
| Author | Cordeiro, R. S. B. | |
| Author | Silva, P. M. R. | |
| Author | Martins, M. A. | |
| Access date | 2019-12-10T13:38:51Z | |
| Available date | 2019-12-10T13:38:51Z | |
| Document date | 2005 | |
| Citation | LAVICH, T. R. et al. A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 38, p. 445-451, 2005. | pt_BR |
| ISSN | 0100-879X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/37746 | |
| Language | eng | pt_BR |
| Publisher | Associação Brasileira de Divulgação Científica | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Hyperalgesia | pt_BR |
| Subject in Portuguese | Teste de Hargreaves | pt_BR |
| Subject in Portuguese | Teste modificado da placa quente | pt_BR |
| Subject in Portuguese | Carragenina | pt_BR |
| Subject in Portuguese | Prostaglandina E2 | pt_BR |
| Subject in Portuguese | Indometacina | pt_BR |
| Title | A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/s0100-879x2005000300016 | |
| Abstract | It is widely accepted that the classical constant-temperature hot-plate test is insensitive to cyclooxygenase inhibitors. In the current study, we developed a variant of the hot-plate test procedure (modified hot-plate (MHP) test) to measure inflammatory nociception in freely moving rats and mice. Following left and right hind paw stimulation with a phlogogen and vehicle, respectively, the animals were placed individually on a hot-plate surface at 51 degrees C and the withdrawal latency for each paw was determined simultaneously in measurements performed at 15, 60, 180, and 360 min post-challenge. Plantar stimulation of rats (250 and 500 microg/paw) and mice (125-500 microg/paw) with carrageenan led to a rapid hyperalgesic response of the ipsilateral paw that reached a plateau from 15 to 360 min after challenge. Pretreatment with indomethacin (4 mg/kg, i.p.) inhibited the phenomenon at all the times analyzed. Similarly, plantar stimulation of rats and mice with prostaglandin E2 (0.5 and 1 microg/paw) also resulted in rapid hyperalgesia which was first detected 15 min post-challenge. Finally, we observed that the MHP test was more sensitive than the classical Hargreaves' test, being able to detect about 4- and 10-fold lower doses of prostaglandin E2 and carrageenan, respectively. In conclusion, the MHP test is a simple and sensitive method for detecting peripheral hyperalgesia and analgesia in rats and mice. This test represents a low-cost alternative for the study of inflammatory pain in freely moving animals. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Hyperalgesia | pt_BR |
| Subject | Hargreaves’ test | pt_BR |
| Subject | Modified hot-plate test | pt_BR |
| Subject | Carrageenan | pt_BR |
| Subject | Prostaglandin E2 | pt_BR |
| Subject | Indomethacin | pt_BR |
| e-ISSN | 1414-431X | |
