| Author | Waghabi, M. C. | |
| Author | Keramidas, M. | |
| Author | Feige, J. J. | |
| Author | Araujo-Jorge, Tania C. | |
| Author | Bailly, S. | |
| Access date | 2019-12-12T15:54:03Z | |
| Available date | 2019-12-12T15:54:03Z | |
| Document date | 2005 | |
| Citation | WAGHABI, M. C. et al. Activation of transforming growth factor b by Trypanosoma cruzi. Celular Microbiology, v. 7, n. 4, p. 511-517, 2005. | pt_BR |
| ISSN | 1462-5814 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/37911 | |
| Language | eng | pt_BR |
| Publisher | Wiley 12 Months | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Transformando o fator de crescimento b | pt_BR |
| Subject in Portuguese | Ativação | pt_BR |
| Subject in Portuguese | TGFbeta | pt_BR |
| Title | Activation of transforming growth factor beta by Trypanosoma cruzi | pt_BR |
| Type | Article | |
| DOI | 10.1111/j.1462-5822.2004.00481.x | |
| Abstract | The anti-inflammatory cytokine, transforming growth factor beta (TGFbeta), plays an important role in Chagas disease, which is caused by the protozoan parasite Trypanosoma cruzi. In the current study, we show that the addition of an anti-TGFbeta antibody inhibited T. cruzi infection of cardiomyocytes, demonstrating the requirement for active endogenous TGFbeta. As TGFbeta is synthesized as a biologically inactive precursor, which is proteolytically processed to yield a mature, active homodimer, we hypothesized that T. cruzi could activate latent TGFbeta. To test this, we added recombinant latent TGFbeta to a TGFbeta-responsive reporter cell line in the presence of T. cruzi. We observed that T. cruzi was able to activate latent recombinant TGFbeta in this cellular model. We then investigated the ability of T. cruzi to activate latent TGFbetain vitro. We found that live T. cruzi, or cytosolic extracts of T. cruzi, activated latent TGFbeta in a dose- and temperature-dependent manner. The agent involved in TGFbeta activation was shown to be thermolabile and hydrophobic. Taken together, our studies demonstrate that T. cruzi directly activates latent TGFbeta. This activation is required for parasite entry into the mammalian cells and is likely to play an important role in modulating the outcome of T. cruzi infection. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Unité INSERM EMI 105. Department of Cellular Responses and Dynamics. Grenoble, France. | pt_BR |
| Affilliation | Unité INSERM EMI 105. Department of Cellular Responses and Dynamics. Grenoble, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Unité INSERM EMI 105. Department of Cellular Responses and Dynamics. Grenoble, France. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Transforming growth factor b | pt_BR |
| Subject | Activation | pt_BR |
| Subject | TGFbeta | pt_BR |
| e-ISSN | 1462-582 | |
| Embargo date | 2022-01-01 | |
