Author | Ribeiro, Vitor Márcio | |
Author | Miranda, Julia Bahia | |
Author | Marcelino, Andreza Pain | |
Author | Andrade, Hélida Monteiro de | |
Author | Reis, Ilka Afonso | |
Author | Cardoso, Mariana Santos | |
Author | Gontijo, Célia Maria Ferreira | |
Author | Paz, Gustavo Fontes | |
Access date | 2019-12-16T12:44:19Z | |
Available date | 2019-12-16T12:44:19Z | |
Document date | 2019 | |
Citation | RIBEIRO, Vitor Márcio et al. Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs. Veterinary Parasitology, v. 274, p. 1-6, 2019. | pt_BR |
ISSN | 0304-4017 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/37960 | |
Description | Andreza Pain Marcelino. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento. | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.vetpar.2019.08.014 | |
Abstract | Visceral leishmaniasis (VL) is a zoonosis caused by the parasite Leishmania infantum and the dog is its main reservoir in rural and urban areas. The diagnosis of infection is mainly based on the presence of anti-Leishmania IgG antibodies in the serum of infected dogs. In this study, the sensitivity and specificity of qualitative rapid tests (RTs) dual path platform (DPP) Bio-Manguinhos, rapid enzyme-linked immunosorbent assay (ELISA) IDEXX, Kalazar Detect and ALERE, as well as quantitative ELISA Bio-Manguinhos and in-house indirect immunofluorescence assay (IFA) tests were analyzed in sera from infected and uninfected dogs. Serial dilutions of the in-house IFA were compared with RTs and ELISA Bio-Manguinhos. The results showed that none of the tests reached 100% sensitivity and specificity. There was no statistical difference between the analyzed RTs. The most sensitive test was the DPP Bio-Manguinhos (97.9%), while the rapid ELISA IDEXX showed higher specificity (100%). In the treatment setting of infected and/or diseased animals, quantitative tests for monitoring the evolution of antibody titers are required, which indicates the maintenance of in-house IFA in animal handling. Furthermore, we demonstrate that the RTs present higher sensitivity in serum samples with superior antibody titers obtained in the in-house IFA. However, the RTs exhibited false negatives in samples with low titers of antibodies. Among the RTs, only the DPP Bio-Manguinhos presented better performance in this situation. Therefore, the use of RTs for the diagnosis of VL in dogs with low titers of antibodies, such as asymptomatic, should be carefully evaluated. | pt_BR |
Affilliation | Santo Agostinho Hospital Veterinário. Belo Horizonte, MG, Brasil / Pontifícia Universidade Católica de Minas Gerais. Escola de Veterinária. Betim, Brazil / Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Ezequiel Dias. Divisão de Epidemiologia e Controle de Doenças. Serviço de Doenças Parasitárias. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Exatas. Departamento de Estatística. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
Subject | Canine leishmaniasis | pt_BR |
Subject | Serological diagnosis | pt_BR |
Subject | Rapid tests | pt_BR |
Subject | Enzyme-linked immunosorbent assay | pt_BR |
Subject | Indirect immunofluorescence assay | pt_BR |
Embargo date | 2020-12-16 | |