Author | Mello, Marco Antônio Gomes | |
Author | Mascarenhas, Rita Elizabeth Moreira | |
Author | Ferraro, Geraldo Argolo | |
Author | Harn Junior, Donald A | |
Author | Castro Filho, Bernardo Galvão | |
Author | Bou-Habib, Dumith Chequer | |
Access date | 2012-03-23T21:31:30Z | |
Available date | 2012-03-23T21:31:30Z | |
Document date | 2005 | |
Citation | MELLO, M.A. et al. Cinhibition of HIV-1 infection by monoclonal antibodies to carbohydrates of Schistosoma mansoni. Medical Microbiology Immunology, v. 194, n.1-2, p. 61-5, jan. 2005. | pt_BR |
ISSN | 0300-8584 | |
URI | https://www.arca.fiocruz.br/handle/icict/3813 | |
Language | eng | pt_BR |
Rights | open access | |
Title | Inhibition of HIV-1 infection by monoclonal antibodies to carbohydrates of Schistosoma mansoni. | pt_BR |
Type | Article | |
Abstract | Patients infected with HIV-1 develop a potent humoral immune response against the virus, but HIV-1 primary isolates are remarkably resistant to neutralizing antibodies. Considering that the envelope glycoprotein of HIV-1 (gp120/41) is heavily glycosylated, we investigated whether anti-carbohydrate antibodies could inhibit HIV-1 infection in vitro. We studied the neutralizing activity of three monoclonal antibodies (mAbs) raised to carbohydrates of Schistosoma mansoni, against seven primary isolates of HIV-1. Assays were performed infecting peripheral blood mononuclear cells from normal donors with viral isolates previously treated with mAbs. Viral strains used were tropic for the coreceptors CCR5, CXCR4, and dual-tropic ones. We found that the anti-glycan mAbs vigorously inhibited HIV-1 infection, regardless of the preferential coreceptor usage of the isolate, in a dose-response manner. Importantly, five isolates were resistant to neutralization by two HIV-1 antibody-positive human sera endowed with potent anti-HIV-1 inhibitory activity. Our findings suggest that carbohydrates of the HIV-1 viral envelope may be a target of an effective humoral immune response elicited by vaccination. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil | pt_BR |
Affilliation | Harvard School of Public Health. Boston, Massachusetts, USA | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | HIV-1 | pt_BR |
Subject | Aids | pt_BR |
Subject | Neutralizing | pt_BR |
Subject | Antibodies | pt_BR |
DeCS | Anticorpos Anti-Helmínticos | pt_BR |
DeCS | Anticorpos Monoclonais | pt_BR |
DeCS | Carboidratos | pt_BR |
DeCS | Infecções por HIV | pt_BR |
DeCS | Schistosoma mansoni | pt_BR |
DeCS | Antígenos de Helmintos | pt_BR |
DeCS | Infecções por HIV | pt_BR |
DeCS | HIV | pt_BR |
DeCS | Testes de Neutralização | pt_BR |
DeCS | Animais | pt_BR |