| Author | Chimelli, L. M. | |
| Author | Valentim, V. C. | |
| Author | Corte-Real, S. | |
| Author | Sarno, Euzenir N. | |
| Author | Jardim, M. R. | |
| Access date | 2019-12-26T11:26:36Z | |
| Available date | 2019-12-26T11:26:36Z | |
| Document date | 2006 | |
| Citation | ANTUNES, S. L. G. et al. An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy. Brazilian Journal of Medical and Biological Research, v. 39, p. 1071-1081, 2006. | pt_BR |
| ISSN | 0100-879X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/38816 | |
| Language | eng | pt_BR |
| Publisher | Associação Brasileira de Divulgação Científica | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Proteína de neurofilamento | pt_BR |
| Subject in Portuguese | Receptor do fator de crescimento nervoso | pt_BR |
| Subject in Portuguese | Proteína básica de mielina | pt_BR |
| Subject in Portuguese | Produto genético 9.5 | pt_BR |
| Subject in Portuguese | Hanseníase neurítica | pt_BR |
| Subject in Portuguese | Proteína S-100 | pt_BR |
| Title | An immunohistochemical, clinical and electroneuromyographic correlative study of the neural markers in the neuritic form of leprosy | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0100-879X2006000800010 | |
| Abstract | The nerve biopsies of 11 patients with pure neuritic leprosy were
submitted to routine diagnostic procedures and immunoperoxidase
staining with antibodies against axonal (neurofilament, nerve growth
factor receptor (NGFr), and protein gene product (PGP) 9.5) and
Schwann cell (myelin basic protein, S-100 protein, and NGFr) markers.
Two pairs of non-adjacent histological cross-sections of the
peripheral nerve were removed for quantification. All the fascicles of
the nerve were examined with a 10X-ocular and 40X-objective lens.
The immunohistochemistry results were compared to the results of
semithin section analysis and clinical and electroneuromyographic
data. Neurofilament staining was reduced in 100% of the neuritic
biopsies. NGFr positivity was also reduced in 81.8%, PGP staining in
100% of the affected nerves, S100 positivity in 90.9%, and myelin
basic protein immunoreactivity in 90.9%. Hypoesthesia was associated
with decreased NGFr (81.8%) and PGP staining (90.9%). Reduced
potential amplitudes (electroneuromyographic data) were found
to be associated with reduced PGP 9.5 (63.6%) and nerve fiber
neurofilament staining (45.4%) by immunohistochemistry and with
loss of myelinated fibers (100%) by semithin section analysis. On the
other hand, the small fibers (immunoreactive dots) seen amid inflammatory
cells continued to be present even after 40% of the larger
myelinated fibers had disappeared. The present study shows an indepth
view of the destructive effects of leprosy upon the expression of
neural markers and the integrity of nerve fiber. The association of
these structural changes with the clinical and electroneuromyographic
manifestations of leprosy peripheral neuropathy was also discusse. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Neurofilament protein | pt_BR |
| Subject | Nerve growth factor receptor | pt_BR |
| Subject | S-100 protein | pt_BR |
| Subject | Myelin basic protein | pt_BR |
| Subject | Gene product 9.5 | pt_BR |
| Subject | Neuritic leprosy | pt_BR |
| e-ISSN | 1414-431X | |
