| Author | Bonaldo, Myrna C. | |
| Author | Garrat, Richard C. | |
| Author | Freire, Marcos S. | |
| Author | Galler, Ricardo | |
| Access date | 2020-01-01T18:43:03Z | |
| Available date | 2020-01-01T18:43:03Z | |
| Document date | 2006 | |
| Citation | BONALDO, Myrna C. et al. Expression of Foreign Protein Epitopes at the Surface of Recombinant Yellow Fever 17D Viruses Based on Three-Dimensional Modeling of Its Envelope Protein. Cell Biochemistry and Biophysics Cell Biochemistry and Biophysics, v. 44, p. 313-324, 2006. | pt_BR |
| ISSN | 1085-9195 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/38926 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Vírus da febre amarela 17D | pt_BR |
| Subject in Portuguese | Estrutura da proteína E | pt_BR |
| Subject in Portuguese | Expressão de epítopo estranho | pt_BR |
| Subject in Portuguese | Atenuação | pt_BR |
| Title | Expression of Foreign Protein Epitopes at the Surface of Recombinant Yellow Fever 17D Viruses Based on Three-Dimensional Modeling of Its Envelope Protein | pt_BR |
| Type | Article | |
| DOI | 10.1385/CBB:44:3:313 | pt_BR |
| Abstract | The yellow fever (YF) 17D vaccine is a live attenuated virus, and its genetic manipulation constitutes a new platform for vaccine development. In this article, we review one of the possible approaches to enable this development, which is the insertion of foreign protein epitopes into different locations of the genome. We describe the three-dimensional (3D) modeling of the YF 17D virus E protein structure based on tick-borne encephalitis (TBE) and the identification of a potential insertion site located at the YF 17D fgloop. Further 3D analysis revealed that it is possible to accommodate inserts of different sizes and amino acid composition in the flavivirus E protein fg loop. We demonstrate that seven YF 17D viruses bearing foreign epitopes that vary in sequence and length show differential growth characteristics in cell culture. The testing of recombinant viruses for mouse neurovirulence suggests that insertions at the 17D E protein fg loop do not compromise the attenuated phenotype of YF 17D virus, further confirming the potential use of this site for the development of new live attenuated 17D virusbased vaccines. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Fisica de São Carlos. São Carlos, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Departamento de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Departamento de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Yellow fever 17D virus | pt_BR |
| Subject | E protein structure | pt_BR |
| Subject | Foreign epitope expression | pt_BR |
| Subject | Attenuation | pt_BR |
