| Author | Cidade, Daniela A. P. | |
| Author | Wermelinger, Luciana S. | |
| Author | Lôbo-Hajdu, Gisele | |
| Author | Dávila, Alberto M. R. | |
| Author | Bon, Cassian | |
| Author | Zingali, Russolina B. | |
| Author | Albano, Rodolpho M. | |
| Access date | 2020-01-02T20:01:54Z | |
| Available date | 2020-01-02T20:01:54Z | |
| Document date | 2006 | |
| Citation | CIDADE, Daniela A. P. et al. Molecular diversity of desintegrin-like domains within metalloproteinase precursors of Bothrops jararaca. Toxicon, v. 48, n. 5, p. 590-599, Oct. 2006. | pt_BR |
| ISSN | 0041-0101 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/38952 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Veneno de serpente | pt_BR |
| Subject in Portuguese | Toxina | pt_BR |
| Subject in Portuguese | Evolução | pt_BR |
| Subject in Portuguese | Análise de purificação e espectrometria de massa | pt_BR |
| Subject in Portuguese | Peptídeos | pt_BR |
| Title | Molecular diversity of disintegrin-like domains within metalloproteinase precursors of Bothrops jararaca | en_US |
| Type | Article | |
| DOI | 10.1016/j.toxicon.2006.07.010 | pt_BR |
| Abstract | Disintegrins are small peptides isolated from the venom of several snake families which act as integrin-antagonists or agonists, interacting with a variety of biological processes mediated by integrins. In this work we describe five new disintegrin-like domains within metalloproteinase precursor sequences, obtained from a Bothrops jararaca venom gland cDNA library. Among the new disintegrin-like domains, four were contained in PIII metalloproteinase precursors, with three of them presenting ECD-motifs and one presenting a new KCD-motif. Moreover, we found three disintegrin-like domains within PII metalloproteinase precursors. Two of them are similar to the already described disintegrins jarastatin and jararacin. The third molecule is unusual, presenting some typical PIII metalloproteinase characteristics but lacking the cysteine-rich domain being, thus, classified as a PII metalloproteinase. Only few reports presented molecules with these characteristics. Sequence analysis suggests that these molecules are intermediate steps between the more ancient PIII and the more recent PII metalloproteinases. We also investigated disintegrin N-terminus diversity in B. jararaca crude venom by purifying jarastatin and jararacin and analyzing them by mass spectrometry. | en |
| Affilliation | Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica. Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Biologia Celular e Genética. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Centre National de la Recherche Scientifique. Paris, France. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica. Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Snake venom | en |
| Subject | Toxin | en |
| Subject | Desintegrin | en |
| Subject | Sructure activity relationships | en |
| Subject | Evolution | en |
| Subject | Purification and mass spectrometry analysis | en |
| e-ISSN | 1879-3150 | pt_BR |
| Embargo date | 2025-01-01 | |
