| Author | Santos, Claudio Cesar Cirne | |
| Author | Teixeira, Valéria L. | |
| Author | Castello-Branco, Luiz Roberto | |
| Author | Frugulhetti, Izabel C. P. P. | |
| Author | Bou-Habib, Dumith Chequer | |
| Access date | 2020-01-03T13:29:06Z | |
| Available date | 2020-01-03T13:29:06Z | |
| Document date | 2006 | |
| Citation | SANTOS, Claudio Cesar Cirne et al. Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii. Planta Medica, v. 72, p. 295-299, 2006. | pt_BR |
| ISSN | 0032-0943 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/38957 | |
| Language | eng | pt_BR |
| Publisher | Georg Thieme Verlag | pt_BR |
| Rights | open access | |
| Subject in Portuguese | HIV-1 | pt_BR |
| Subject in Portuguese | AIDS | pt_BR |
| Subject in Portuguese | Dictyota pfaffii | pt_BR |
| Title | Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii | pt_BR |
| Type | Article | |
| DOI | 10.1055/s-2005-916209 | |
| Abstract | We describe in this paper that the dolabellane diterpene 8,10,18-trihydroxy-2,6-dolabelladiene (3), isolated from the marine algae Dictyota pfaffii, inhibits the HIV-1 infection in human primary cells and tumor cell lines. We initially observed that compound 3 inhibited the activity of a purified HIV-1 enzyme reverse transcriptase (RT) in a dose-dependent manner, with an IC (50) value of 16.5 +/- 4.3 microM. Next, we found that compound 3 inhibited HIV-1 infection by an R5-tropic isolate in peripheral blood mononuclear cells (PBMCs) in a dose-dependent manner with an EC (50) value of 8.4 +/- 2.8 microM. The replication of HIV-1 isolates presenting distinct tropism for chemokine receptors was also inhibited, as analyzed in PBMCs or U87 cells infected with R5-, X4- or R5X4-tropic isolates. Likewise, compound 3 blocked HIV-1 infection in macrophages by R5 and R5X4 viruses in a dose-dependent manner with EC (50) values of 1.7 +/- 0.6 microM and 1.85 +/- 0.75 microM, respectively. Compound 3 sustained antiretroviral activity even when added to HIV-1-infected Sup-T1 cells at 12 h after infection, suggesting that, as well as inhibiting HIV-1 RT, it also blocks HIV-1 replication at a post transcriptional step. Our results support further investigations on compound 3 pharmacokinetics and we propose that this diterpene could be considered as a potential compound for HIV-1 therapy. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Laboratório de Virologia Molecular. Niterói, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Departamento de Biologia Marinha. Laboratório de Biologia Marinha. Niteói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Laboratório de Virologia Molecular. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Dictyota pfaffii | pt_BR |
| Subject | HIV-1 | pt_BR |
| Subject | AIDS | pt_BR |
| Subject | Diterpenes | pt_BR |
| Subject | Dolabellane | pt_BR |
| e-ISSN | 1439-0221 | |
