| Author | Guimarães Júnior, Milton Henriques | |
| Author | Ferrari, Teresa Cristina Abreu | |
| Author | Carvalho, Andréa Teixeira de | |
| Author | Moreira, Marcela de Lima | |
| Author | Santos, Lorena Júnia de Souza | |
| Author | Silva, Matheus Fernandes Costa | |
| Author | Coelho, Rodrigo Matos Pinto | |
| Author | Pinto, Pedro Henrique Oliveira Murta | |
| Author | Ris, Tijmen Hermen | |
| Author | Salles, Jonathas Teixeira | |
| Author | Passos, Lívia Silva Araújo | |
| Author | Nunes, Maria Carmo Pereira | |
| Access date | 2020-01-03T13:51:24Z | |
| Available date | 2020-01-03T13:51:24Z | |
| Document date | 2019 | |
| Citation | GUIMARÃES JÚNIOR, Milton Henriques et al. Cell-derived microvesicles in infective endocarditis: Role in diagnosis and potential for risk stratification at hospital admission. Journal of Infection, v. 79, n. 2, p. 101-107, 2019. | pt_BR |
| ISSN | 0163-4453 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/38960 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Cell-derived microvesicles in infective endocarditis: Role in diagnosis and potential for risk stratification at hospital admission | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jinf.2019.06.005 | |
| Abstract | OBJECTIVES: To characterize the plasmatic profile of cell-derived microvesicles (MVs) at diagnosis and during the treatment of patients with infective endocarditis (IE). METHODS: Blood samples from 57 patients with IE were obtained on 3 consecutive moments: upon admission (T0), at 2 weeks (T1), and at the end of treatment (T2), and were compared with 22 patients with other bacterial infections. MPs were measured by flow cytometry and labeled for specific cell markers of CD45 (leukocytes), CD66b (neutrophils), CD14 (monocytes), CD41a (platelets), CD51 (endothelial cells), CD3 (T lymphocyte) and CD235a (erythrocytes). RESULTS: MVs from platelets (pltMVs), leukocytes (leukMVs), neutrophils (neutMVs), monocytes (monoMVs) and lymphocytes (lymphMVs) were significantly more elevated in the patients with IE, compared to the patients with other bacterial infections, despite comparable age, sex, blood counts and C-reactive protein levels. MVs values revealed a relatively stable pattern over time in IE, except for a significant increase in leukMVs and neutMVs in T1. LeukMVs (p = 0.011), neutMVs (p = 0.010), monoMVs (p = 0.016) and lymphMVs (p = 0.020), measured at admission, were significantly higher in IE patients that died during hospitalization in comparison with those that survived. In a multivariable analyses, the levels of neutMVs remained as an independent factor associated with mortality (odds ratio 2.203; 95% confidence interval 1.217 - 3.988; p = 0.009), adjustment for heart failure during the treatment. CONCLUSIONS: Plasma levels of pltMVs, leukMVs, neutMVs, monoMVs and lymphMVs were significantly more elevated in patients with IE than in patients with other bacterial infections at hospital admission. Furthermore, neutMVs at admission have been identified as an independent predictor of mortality in patients with IE. Thus, cell derived MPs may become an important tool in the differential diagnosis and mortality risk assessment early in the course of IE suspected cases. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical e Departamento de Clínica Médica. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical e Departamento de Clínica Médica. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração, Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração, Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração, Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração, Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical e Departamento de Clínica Médica. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical e Departamento de Clínica Médica. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Erasmus University Rotterdam. Erasmus MC, Netherlands. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical e Departamento de Clínica Médica. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical e Departamento de Clínica Médica. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical e Departamento de Clínica Médica. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Bacterial infections | pt_BR |
| Subject | Cell-derived microparticles | pt_BR |
| Subject | mortality | pt_BR |
| Subject | Infective endocarditis | pt_BR |
| Embargo date | 2035-01-01 | |
