| Author | Fonseca, Denise Morais da | |
| Author | Silva, Celio Lopes | |
| Author | Wowk, Pryscilla Fanini | |
| Author | Paula, Marina Oliveira e | |
| Author | Ramos, Simone Gusmão | |
| Author | Horn, Cynthia | |
| Author | Marchal, Gilles | |
| Author | Bonato, Vânia Luiza Deperon | |
| Access date | 2020-01-08T13:10:36Z | |
| Available date | 2020-01-08T13:10:36Z | |
| Document date | 2009 | |
| Citation | FONSECA, Denise Morais da et al. Mycbacterium tuberculosis culture filtrate proteins plus CpG Oligodeoxynucleotides confer protection to Mycobacterium bovis BCG-primed mice by inhibiting interleukin-4 secretion. Infection and Immunity, v. 77, n. 12, p. 5311-5321, Dec. 2009. | pt_BR |
| ISSN | 0019-9567 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/39057 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | pt_BR |
| Title | Mycobacterium tuberculosis culture filtrate proteins plus CpG-ODN confer protection to BCG primed mice by inhibiting IL-4 secretion | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1128/IAI.00580-09 | |
| Abstract | Culture filtrate proteins (CFP) are potential targets for tuberculosis vaccine development. We previously showed that despite the high level of gamma interferon (IFN- γ) production elicited by homologous immunization with CFP plus CpG oligodeoxynucleotides (CFP/CpG), we did not observe protection when these mice were challenged with Mycobacterium tuberculosis. In order to use the IFN- γ-inducing ability of CFP antigens, in this study we evaluated a prime-boost heterologous immunization based on CFP/CpG to boost Mycobacterium bovis BCG vaccination in order to find an immunization schedule that could induce protection. Heterologous BCG-CFP/CpG immunization provided significant protection against experimental tuberculosis, and this protection was sustained during the late phase of infection and was even better than that conferred by a single BCG immunization. The protection was associated with high levels of antigen-specific IFN- γ and
interleukin-17 (IL-17) and low IL-4 production. The deleterious role of IL-4 was confirmed when IL-4 knockout
mice vaccinated with CFP/CpG showed consistent protection similar to that elicited by BCG-CFP/CpG heterologous immunization. These findings show that a single dose of CFP/CpG can represent a new strategy to boost the protection conferred by BCG vaccination. Moreover, different immunological parameters, such as
IFN- γ and IL-17 and tightly regulated IL-4 secretion, seem to contribute to the efficacy of this tuberculosis
vaccine. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Núcleo de Pesquisas em Tuberculose. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Núcleo de Pesquisas em Tuberculose. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Núcleo de Pesquisas em Tuberculose. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Núcleo de Pesquisas em Tuberculose. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Patologia. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Fundação Osvaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Imunologia e Imunogenética. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Institut Pasteur. Laboratoire d’Immunothérapie. Paris, France. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Núcleo de Pesquisas em Tuberculose. Ribeirão Preto, SP, Brasil. | pt_BR |
| Subject | Mycobacterium tuberculosis | pt_BR |
| Subject | Culture Filtrate Proteins | pt_BR |
| Subject | Tuberculosis | pt_BR |
| Subject | Vaccine | pt_BR |
| e-ISSN | 1098-5522 | |
| Embargo date | 2020-07-08 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
