Author | Diz, Mariângela S. S. | |
Author | Carvalho, André O. | |
Author | Rodrigues, Rosana | |
Author | Ferreira, Ana Gisele C. Neves | |
Author | Cunha, Maura da | |
Author | Alves, Elias Walter | |
Author | Façanha, Anna L. Okorokova | |
Author | Oliveira, Marco Antônio | |
Author | Perales, Jonas | |
Author | Machado, Olga L. T. | |
Author | Gomes, Valdirene M. | |
Access date | 2020-01-16T13:09:08Z | |
Available date | 2020-01-16T13:09:08Z | |
Document date | 2006 | |
Citation | DIZ, Mariângela S. S. et al. Antimicrobial peptides from chilli pepper seeds causes yeast plasma membrane permeabilization and inhibits the acidification of the medium by yeast cells. Biochimica et Biophysica Acta, v. 1760, p. 1323-1332, 2006. | pt_BR |
ISSN | 0006-3002 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/39254 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | |
Subject in Portuguese | Capsicum annuum | pt_BR |
Subject in Portuguese | Saccharomyces cerevisiae | pt_BR |
Subject in Portuguese | Candida albicans | pt_BR |
Subject in Portuguese | Proteína de transferência lipídica | pt_BR |
Subject in Portuguese | Permeabilização de membranas | pt_BR |
Subject in Portuguese | Péptido antimicrobiano de plantas | pt_BR |
Title | Antimicrobial peptides from chilli pepper seeds causes yeast plasma membrane permeabilization and inhibits the acidification of the medium by yeast cells | pt_BR |
Type | Article | |
DOI | 10.1016/j.bbagen.2006.04.010 | |
Abstract | During the last few years, a growing number of cysteine-rich antimicrobial peptides has been isolated from plants and particularly from seeds.
It has become increasingly clear that these peptides play an important role in the protection of plants against microbial infection. In this work,
proteins from chilli pepper (Capsicum annuum L.) seeds were extracted in phosphate buffer, pH 5.4 and peptides purification were performed by
employing ion-exchange chromatographies on DEAE, CM-Sepharose, Sephacryl S-100 and reverse phase in HPLC. Three peptide enriched
fractions, namely F1, F2 and F3, were obtained after the CM-Sepharose chromatography. The F1 fraction, mainly composed of three peptides
ranging from 6 to 10 kDa, was submitted to N-terminal amino acid sequencing. The closer to 10 kDa peptide showed high sequence homology to
lipid transfer proteins (LTPs) previously isolated from others seeds. F1 fraction exhibited strong fungicidal activity against Candida albicans,
Saccharomyces cerevisiae and Schizosaccharomyces pombe and also promoted several morphological changes to C. albicans, including the
formation of pseudohyphae, as revealed by scanning electron micrography. F1 fraction also reduced the glucose stimulated acidification of the
medium mediated by H+-ATPase of S. cerevisiae cells in a dose-dependent manner and caused the permeabilization of yeast plasma membrane to
the dye SYTOX Green, as verified by confocal laser microscopy. | pt_BR |
Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Campos dos Goytacazes, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Campos dos Goytacazes, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Norte Fluminense. Centro de Ciências e Tecnologias, Agropecuárias. Campos dos Goytacazes, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade do Vale do Paraíba. Instituto de Pesquisa e Desenvolvimento. São José dos Campos, SP, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Campos dos Goytacazes, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Campos dos Goytacazes, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Campos dos Goytacazes, RJ, Brasil / Universidade do Vale do Paraíba. Instituto de Pesquisa e Desenvolvimento. São José dos Campos, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Campos dos Goytacazes, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Campos dos Goytacazes, RJ, Brasil. | pt_BR |
Subject | Lipid transfer protein | pt_BR |
Subject | Membrane permeabilization | pt_BR |
Subject | Capsicum annuum | pt_BR |
Subject | Plant antimicrobial peptide | pt_BR |
Subject | Saccharomyces cerevisiae | pt_BR |
Subject | Candida albicans | pt_BR |
Embargo date | 2022-01-01 | |