| Author | Gonzalez, M. S. | |
| Author | Hamedi, A. | |
| Author | Cunha, J. M. Albuquerque | |
| Author | Nogueira, N. F. S. | |
| Author | Souza, W. de | |
| Author | Ratcliffe, N. A. | |
| Author | Azambuja, P. | |
| Author | Garcia, E. S. | |
| Author | Mello, C. B. | |
| Access date | 2020-01-21T12:03:08Z | |
| Available date | 2020-01-21T12:03:08Z | |
| Document date | 2006 | |
| Citation | GONZALEZ, M. S. et al. Antiserum against perimicrovillar membranes and midgut tissue reduces the development of Trypanosoma cruzi in the insect vector, Rhodnius prolixus. Experimental Parasitology, v. 114, n. 4, p. 297-304, Dec. 2006. | pt_BR |
| ISSN | 0014-4894 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/39335 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Anti-soro | pt_BR |
| Subject in Portuguese | Membranas peritróficas e perimicrovilares | pt_BR |
| Subject in Portuguese | Protozoários | pt_BR |
| Title | Antiserum against perimicrovillar membranes and midgut tissue reduces the development of Trypanosoma cruzi in the insect vector, Rhodnius prolixus | en_US |
| Type | Article | |
| DOI | 10.1016/j.exppara.2006.04.009 | pt_BR |
| Abstract | Antiserum raised against Rhodnius prolixus perimicrovillar membranes (PMM) and midgut tissue interfered with the midgut structural organization and reduced the development of Trypanosoma cruzi in the R. prolixus insect vector. SDS-PAGE and Western blot analyses confirmed the specific recognition of midgut proteins by the antibody. Feeding, mortality, molt, and oviposition of the insects were unaffected by feeding with the antiserum. However, the eclosion of the eggs were reduced from R. prolixus females treated with antiserum. Additionally, in vivo evaluation showed that after oral treatment with the antiserum, the intensity of infection with the Dm-28c clone of T. cruzi decreased in the digestive tract of fifth-instar nymphs and in the excretions of R. prolixus adults. These results suggest that the changes observed in the PMM organization in the posterior midgut of R. prolixus may not be important for triatomine survival but the antiserum acts as a transmission-reduction vaccine able to induce significant decreases in T. cruzi infection in the vector. | en |
| Affilliation | Universidade Federal Fluminense. Departamento de Biologia Geral. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Departamento de Biologia Geral. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacazes, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense. Centro de Biociências e Biotecnologia. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacazes, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of Wales. School of Biological Sciences. Swansea, United Kingdom. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Departamento de Biologia Geral. Niterói, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi (protozoan) | en |
| Subject | Rhodnius prolixus | en |
| Subject | Peritrophic and perimicrovillar membranes | en |
| Subject | Antiserum | en |
| e-ISSN | 1090-2449 | pt_BR |
| Embargo date | 2022-01-01 | |
