| Author | Neves, Renata Heisler | |
| Author | Alencar, Alba Cristina Miranda de Barros | |
| Author | Aguila, Marcia Barbosa | |
| Author | Lacerda, Carlos Alberto Mandarim de | |
| Author | Silva, José Roberto Machado | |
| Author | Gomes, Delir Corrêa | |
| Access date | 2020-01-23T15:33:18Z | |
| Available date | 2020-01-23T15:33:18Z | |
| Document date | 2006 | |
| Citation | NEVES, Renata Heisler et al. Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet. International Journal of Morphology, v. 24, n. 4, p. 625-632, 2006. | pt_BR |
| ISSN | 0717-9367 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/39470 | |
| Language | eng | pt_BR |
| Publisher | Desconhecido | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Hipercolesterolemia | pt_BR |
| Subject in Portuguese | Modelo experimental | pt_BR |
| Subject in Portuguese | Ratos | pt_BR |
| Subject in Portuguese | Colesterol | pt_BR |
| Subject in Portuguese | Dieta | pt_BR |
| Subject in Portuguese | Fígado | pt_BR |
| Title | Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet | pt_BR |
| Alternative title | Alteraciones Somáticas Bioquímicas y hepáticas en un Tipo de Ratas Alimentadas Crónicamente con Dieta Alta en Grasas | pt_BR |
| Type | Article | |
| DOI | 10.4067/S0717-95022006000500018 | |
| Abstract | This study evaluated whether a high fat diet (HFC group) induces overweight, hepatic steatosis and plasma lipoproteins level alteration compared to standard chow diet (SC group). Female mice were submitted to each diet over 6 months. Body mass and food intake were evaluated weekly throughout the experiment. Total cholesterol, TG, LDL-c, HDL-c and VLDL-c were analyzed. Mice were sacrificed to remove liver, spleen, heart and intestine. The volume of the organs was determined according to the submersion method. Fixed livers were embedded in paraffin and stained with hematoxylin and eosin and Masson’s trichrome. The analysis used a
video microscope system and a test-system with 42 test-points. The volume density was estimated for hepatocytes, steatosis and sinusoids. Animals fed HFC had smaller chow intake than SC group. HFC group presented body mass greater than SC. Animals fed HFC showed heavier liver and spleen and lighter intestine than SC (p<0.05), heart mass was not significant between groups. Plasma lipoproteins differed between groups (p<0.05) except VLDL-c and TG fractions. The liver structure was without major alteration in SC group however, HFC mice group showed different degrees of fatty degeneration with micro- and macrovesicular steatosis dispersed in all liver with typical peri-cellular/peri-sinusoidal fibrosis. The quantitative study showed significant (p<0.05) volume density reduction for hepatocytes and sinusoids. In conclusion, our results clearly show that hepatic steatosis can be induced in mouse by such a fat-rich diet without any toxin ingestion, alimentary deficiency and genes depletion. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Roberto Lascasas Porto. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Roberto Lascasas Porto. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia. Laboratório de Morfometria e Morfologia Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia. Laboratório de Morfometria e Morfologia Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Roberto Lascasas Porto. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Hypercholesterolemia | pt_BR |
| Subject | Experimental model | pt_BR |
| Subject | Mice | pt_BR |
| Subject | Cholesterol | pt_BR |
| Subject | Diet | pt_BR |
| Subject | Liver | pt_BR |
| e-ISSN | 0717-9502 | |
