| Author | Paulino, Niraldo | |
| Author | Teixeira, Cristiane | |
| Author | Martins, Regiane | |
| Author | Scremin, Amarilis | |
| Author | Dirsch, Verena M. | |
| Author | Vollmar, Angelika M. | |
| Author | Abreu, Sheila R. L. | |
| Author | Castro, Solange L. de | |
| Author | Marcucci, Maria Cristina | |
| Access date | 2020-01-29T16:49:59Z | |
| Available date | 2020-01-29T16:49:59Z | |
| Document date | 2006 | |
| Citation | PAULINO, Niraldo et al. Evaluation of the analgesic and anti-inflammatory effects of a Brazilian green propolis. Planta Medica, v. 72, p. 899-906, 2006. | pt_BR |
| ISSN | 0032-0943 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/39600 | |
| Language | eng | pt_BR |
| Publisher | Georg Thieme Verlag | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Òxifo nítrico | pt_BR |
| Subject in Portuguese | Própolis tipificada | pt_BR |
| Subject in Portuguese | Efeito anti-inflamatório | pt_BR |
| Subject in Portuguese | Efeito analgésico | pt_BR |
| Title | Evaluation of the analgesic and anti-inflammatory effects of a Brazilian green propolis | pt_BR |
| Type | Article | |
| DOI | 10.1055/s-2006-947185 | |
| Abstract | Phamacological activities of a standard ethanol extract G1 from Brazilian green propolis, typified as BRP1, was evaluated in mouse models of pain and inflammation. Intraperitoneal injection ( I. P.) of G1 inhibited acetic acid-induced abdominal constrictions with an ID (50) = 0.75 +/- 0.05 mg/kg, and in the formalin test the ID (50) values were 0.85 +/- 0.07 mg/kg and 13.88 +/- 1.12 mg/kg, respectively, for the neurogenic and inflammatory phases. The extract was ineffective when assessed in the hot-plate assay. In serotonin-induced paw edema, G1 led to a maximal inhibition (MI) of 51.6 % after 120 min when administered I. P. and of 36 % after 15 min by the oral route ( O. R.). When the inflammatory agent was complete Freund's adjuvant, inhibition of paw edema was also observed after administration of the extract by both routes. In the capsaicin-induced ear edema the ID (50) values were 1.09 +/- 0.08 mg/kg ( I. P.) and 10.00 +/- 0.90 mg/kg ( O. R.). In the acute carrageenan-induced inflammatory reaction induced by carrageenan, G1 reduced the number of neutrophils in the peritoneal cavity with IC (50) values of 0.72 +/- 0.08 mg/kg and 4.17 +/- 0.50 mg/kg, by I. P. or O. R. administration, with a preferential migration of polymorphonuclear neutrophils. IN VITRO, G1 decreased nitric oxide production in LPS-stimulated RAW 264.7 cells (IC (50) = 41.60 microg/mL), and also the luciferase activity in TNF-alpha-stimulated HEK 293 cells transfected with NF-kappaB-luciferase reporter gene driven by the nuclear factor kappaB (NF-kappaB) (IC (50) = 200 microg/mL). This extract, which at low concentrations induces anti-inflammatory and analgesic effects in mouse models, presents a high content of flavonoids, known to inhibit inducible NOS (iNOS) activity. These data taken together led us to reinforce the hypothesis in the literature that the anti-inflammatory effect of propolis may be a due to inhibition of iNOS gene expression, through interference with NF-kappaB sites in the iNOS promoter. | pt_BR |
| Affilliation | Universidade Sul de Santa Catarina. Grupo de Pesquiisa e Desenvolvimento de Biofármacos. Tubarão< SC, Brasil. | pt_BR |
| Affilliation | Universidade Sul de Santa Catarina. Grupo de Pesquiisa e Desenvolvimento de Biofármacos. Tubarão, SC, Brasil. | pt_BR |
| Affilliation | Universidade Sul de Santa Catarina. Grupo de Pesquiisa e Desenvolvimento de Biofármacos. Tubarão, SC, Brasil. | pt_BR |
| Affilliation | Universidade Sul de Santa Catarina. Grupo de Pesquiisa e Desenvolvimento de Biofármacos. Tubarão, SC, Brasil. | pt_BR |
| Affilliation | University of Munich. Institute of Pharmacy. Munich, Germany, | pt_BR |
| Affilliation | University of Munich. Institute of Pharmacy. Munich, Germany, | pt_BR |
| Affilliation | Nectar Pharmaceuticals. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Bandeirante. Departamento de Pesquisa e Extensão. Faculdade de Farmácia. São Paulo, SP, Brasil. | pt_BR |
| Subject | Typified propolis | pt_BR |
| Subject | Analgesic effect | pt_BR |
| Subject | Anti-inflammatory effect | pt_BR |
| Subject | NF-xB factor | pt_BR |
| Subject | Nitric oxid | pt_BR |
| e-ISSN | 1439-0221 | |
