| Author | Peirano, Gisele | |
| Author | Agersø, Yvonne | |
| Author | Aarestrup, Frank Møller | |
| Author | Reis, Eliane Moura Falavina dos | |
| Author | Rodrigues, Dália dos Prazeres | |
| Access date | 2020-01-29T17:00:56Z | |
| Available date | 2020-01-29T17:00:56Z | |
| Document date | 2006 | |
| Citation | PEIRANO, Gisele et al. Occurrence of integrons and antimicrobial resistance genes among Salmonella enterica from Brazil. Journal of Antimicrobial Chemotherapy, v. 58, p. 305-309, June 2006. | pt_BR |
| ISSN | 0305-7453 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/39601 | |
| Language | eng | pt_BR |
| Publisher | Oxford University Press | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Salmonella | pt_BR |
| Subject in Portuguese | Determinantes de resistência | pt_BR |
| Subject in Portuguese | Fitas genéticas | pt_BR |
| Title | Occurrence of integrons and antimicrobial resistance genes among Salmonella enterica from Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1093/jac/dkl248 | |
| Abstract | Objectives: To determine the occurrence of antimicrobial resistance genes and role of integrons among 135 antimicrobial-resistant Salmonella enterica from Brazil. Methods: The presence of antimicrobial resistance genes, class 1 and 2 integrons and gene cassettes was analysed by PCR and sequencing. The genetic location of class 1integrons was determinedin 25 isolates by hybridization and plasmid transfer experiments. Results: Fifty-five of the isolates were positive for class 1 integrons. Integron-positive isolates represented 17 different serovars and were mainly from human (n = 28) and animal (n = 13) sources. The gene cassette arrangements could be determined in 51 of the positive isolates, which harboured one [dfrA22, aadA1 or orf3 (putative trimethoprim resistance)], two [aadA1-dfrA1, aac(60
)-Ib-orf1 (unknown function) or aacA4-aadA1], three [dfrA15b-cmlA4-aadA2, orf2 (unknown function)-dfrA5-orfD] or four [orf4-aacA4-blaOXA-30 (interrupted by an IS1 element)-aadA1] cassettes in their variable region. Only one isolate harboured a class 2 integron with the gene cassette array dfrA1-sat-aadA1. Several integron unrelated resistance genes were also detected in the isolates. Sulphonamide resistance was primarily mediated by sul2 and sul3, tetracycline resistance by tet(B) and tet(A), chloramphenicol resistance by catA1, streptomycin resistance by strA and ampicillin resistance by blaTEM. blaCTX and blaCMY-2 were found in cephalosporin-resistant isolates. Mating and hybridization experiments demonstrated that a high-molecular-weight plasmid mediated the gene transfer of integrons and additional resistance determinants. Conclusions: The present study revealed that integron-mediated resistance genes contributed to the multiresistance phenotype observed in the isolates, but most resistance genes were located outside the integron structure, as independent genes. However, they might be located on the same conjugative plasmid. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Danish Institute for Food and Veterinary Research. WHO Collaborating Centre for Antimicrobial Resistance in Foodborne Pathogens. Copenhagen, Denmark. | pt_BR |
| Affilliation | Danish Institute for Food and Veterinary Research. WHO Collaborating Centre for Antimicrobial Resistance in Foodborne Pathogens. Copenhagen, Denmark. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Salmonella | pt_BR |
| Subject | Gene cassettes | pt_BR |
| Subject | Resistance determinants | pt_BR |
| e-ISSN | 1460-2091 | |
| Embargo date | 2025-01-01 | |
