| Author | Silva, Anna Christina Tojal da | |
| Author | Cupolillo, Elisa | |
| Author | Volpini, Ângela Cristina | |
| Author | Almeida, Roque | |
| Author | Romero, Gustavo Adolfo Sierra | |
| Access date | 2020-02-06T10:48:53Z | |
| Available date | 2020-02-06T10:48:53Z | |
| Document date | 2006 | |
| Citation | SILVA, Anna Christina Tojal da et al. Species diversity causing human cutaneous leishmaniasis in Rio Branco, state of Acre, Brazil. Tropical Medicine and Internatiional Health, v. 11, n. 9, p. 1388-1398, Sept. 2006. | pt_BR |
| ISSN | 1360-2276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/39756 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Leishmaniose cutãnea | pt_BR |
| Subject in Portuguese | Diversidade genética | pt_BR |
| Subject in Portuguese | Amazônia Ocidental Brasileira | pt_BR |
| Title | Species diversity causing human cutaneous leishmaniasis in Rio Branco, state of Acre, Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1111/j.1365-3156.2006.01695.x | |
| Abstract | Information on Leishmania species diversity in western Brazilian Amazon and the clinical
picture of human cutaneous leishmaniasis it causes is scarce. We describe clinical findings, diagnostic
procedures and identification of Leishmania species in patients from that region.
methods The sample consisted of 50 patients, prospectively evaluated for epidemiological and clinical
characteristics by means of a structured questionnaire. Conventional and molecular tools were applied
to confirm the parasitological diagnosis and identify the species responsible for the disease.
results Patients were predominantly male (76.5%) and living in rural areas. Median average age was
1 18 years and median average disease evolution was 8 weeks. For the diagnostic procedures of leishmanin
skin test, direct visualization of amastigotes in dermal scrapings and parasite culture of aspirates of
the ulcer border were positive for 98%, 52% and 34%, respectively. Molecular methods applied to
DNA extracted from skin biopsies of the 50 patients yielded 100%, 82% and 44% positivity by PCR
minicircle kDNA, PCR-RFLP ITS1rDNA and PCR-glucose-6-phosphate (G6P), respectively. Fourteen
samples from 13 patients were successfully isolated and identified. Multilocus enzyme electrophoresis,
PCR-RFLP ITS1rDNA and PCR-G6P permitted identification of the Leishmania species responsible for
the aetiology of American tegumentary leishmaniasis in 60% of the examined patients: 16 Leishmania
(Viannia) braziliensis, 12 Leishmania (Viannia) lainsoni, 1 Leishmania (Viannia) guyanensis and 1
putative hybrid of Leishmania (Viannia) naiffi and L. (V.) lainsoni. | pt_BR |
| Affilliation | Universidade de Brasília. Campus Darcy Ribeiro. Núcleo de Medicina Tropical. Brasília, DF, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Pesquisas em Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Pesquisas em Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgar Santos. Serviço de Imunologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade de Brasília. Campus Darcy Ribeiro. Núcleo de Medicina Tropical. Brasília, DF, Brasil | pt_BR |
| Subject | Cutaneous leishmaniasis | pt_BR |
| Subject | Leishmania (Viannia) braziliensis | pt_BR |
| Subject | Leishmania (Viannia) lainsoni | pt_BR |
| Subject | Leishmania (Viannia) naiffi | pt_BR |
| Subject | Western Brazilian Amazon | pt_BR |
| Subject | Genetic diversity | pt_BR |
| e-ISSN | 1365-3156 | |
| Embargo date | 2025-01-01 | |
| xmlui.metadata.dc.subject.ods | 10 Redução das desigualdades | |
