| Author | Torres, Kátia Luz | |
| Author | Figueiredo, Daniel Vasquez | |
| Author | Zalls, Mariano Gustavo | |
| Author | Daniel-Ribeiro, Cláudio Tadeu | |
| Author | Alecrim, Wilson | |
| Author | Cruz, Maria de Fátima Ferreira da | |
| Access date | 2020-02-06T11:23:11Z | |
| Available date | 2020-02-06T11:23:11Z | |
| Document date | 2006 | |
| Citation | TORRES, Kátia Luz et al. Standardization of a very specific and sensitive single PCR for detection of Plasmodium vivax in low parasitized individuals and its usefulness for screening blood donors. Parasitology Research, v. 98, p. 519-524, 2006. | pt_BR |
| ISSN | 0932-0113 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/39758 | |
| Language | eng | pt_BR |
| Publisher | Springer Verlag (Germany) | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Plasmodium vivax | pt_BR |
| Subject in Portuguese | Padronização | pt_BR |
| Subject in Portuguese | Reação em cadeia da polimerase | pt_BR |
| Subject in Portuguese | Doadores de sangue | pt_BR |
| Subject in Portuguese | Indivíduos com baixo parasitismo | pt_BR |
| Title | Standardization of a very specific and sensitive single PCR for detection of Plasmodium vivax in low parasitized individuals and its usefulness for screening blood donors | pt_BR |
| Type | Article | |
| DOI | 10.1007/s00436-005-0085-8 | |
| Abstract | Here, we describe the standardization of a very
sensitive and specific single Plasmodium vivax polymerase
chain reaction (PCR) and its usefulness for diagnosis and
screening procedures when a Plasmodium falciparum PCR
was also utilized. The P. vivax PCR sensitivity threshold
was 0.019 parasites per microliter, and a PCR fragment was
only detected when P. vivax DNA was present. Among the
11 febrile patients with negative parasitological examination
that attended the malaria service of Fundação de
Medicina Tropical do Amazonas, we diagnosed one P.
vivax malaria by PCR. Among the 286 individuals
considered suitable for blood donation, we also detected
by PCR an individual with P. vivax malaria, and conversely,
we did not detect any malaria infection in blood donor
candidates considered unsuitable due to its past malaria
history. We conclude that PCR is the method of choice for
low-parasitized individuals and could therefore represent a
complementary tool to safely rescue blood donor candidates
considered unsuitable on the basis of malaria history. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil / Fundação de Hematologia e Hemoterapia do Amazonas. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Biofísica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Instituto de Medicina Tropical do Amazonas. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Plasmodium vivax | pt_BR |
| Subject | PCR | pt_BR |
| Subject | Parasitized individuals | pt_BR |
| Subject | Blood donors | pt_BR |
| Subject | Standardization | pt_BR |
| e-ISSN | 1432-1955 | |
| Embargo date | 2025-01-01 | |
