| Author | Adade, Camila M. | |
| Author | Castro, Solange L. de | |
| Author | Soares, Maurilio J. | |
| Access date | 2020-02-06T15:10:23Z | |
| Available date | 2020-02-06T15:10:23Z | |
| Document date | 2007 | |
| Citation | ADADE, Camila M.; CASTRO, Solange L. de; SOARES, Maurilio J. Ultrastructural localization of Trypanosoma cruzi lysosomes by aryl sulphatase cytochemistry. Micron, v. 38, p. 252-256, 2006. | pt_BR |
| ISSN | 0968-4328 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/39774 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Crithidia fasciculata | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Lisosomos | pt_BR |
| Subject in Portuguese | Ultraestrutura | pt_BR |
| Subject in Portuguese | Histocitoquímica | pt_BR |
| Title | Ultrastructural localization of Trypanosoma cruzi lysosomes by aryl sulphatase cytochemistry | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.micron.2006.05.002 | |
| Abstract | Lysosomes of trypanosomatid protozoa are poorly known. In this work we have cytochemically detected the lysosomal enzyme aryl sulphatase
in the trypanosomatids Trypanosoma cruzi and Crithidia fasciculata, by using p-nitrocatecholsulphate as substrate. Positive reaction was located
exclusively inside membrane-bound cytoplasmic vesicles distributed throughout the cell body. Electron-dense reaction was either dispersed
homogeneously through the vesicular matrix or located at the vesicle periphery, apposed to the membrane, with fine granular deposits occasionally
found at the vesicular matrix. Trypomastigote and epimastigote forms of T. cruzi lacked electron-dense deposits at the plasma membrane, thus
indicating that aryl sulphatase was not secreted to the environment. Furthermore, no positive reaction was detected in epimastigote reservosomes,
which are organelles considered as pre-lysosomal compartments. Thus, our data show that reservosomes and lysosomes are organelles that can be
distinguished by the cytochemical localization of aryl sulphatase in T. cruzi epimastigotes and trypomastigotes. Positive reaction in cytoplasmic
vesicles of C. fasciculata choanomastigotes confirmed the specificity of the reaction for lysosomes in other trypanosomatid species. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular de Microrganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular de Microrganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Aryl sulphatase | pt_BR |
| Subject | Crithidia fasciculata | pt_BR |
| Subject | Cytochemistry | pt_BR |
| Subject | Lysosome | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Ultrastructure | pt_BR |
| e-ISSN | 1878-4291 | |
| Embargo date | 2025-01-01 | |
