| Author | Araújo, Catarina A. C. | |
| Author | Cabello, Pedro H. | |
| Author | Jansen, Ana Maria | |
| Access date | 2020-02-06T15:24:14Z | |
| Available date | 2020-02-06T15:24:14Z | |
| Document date | 2007 | |
| Citation | ARAÚJO, Catarina A. C.; CABELLO, Pedro H.; JANSEN, Ana Maria. Growth behaviour of two Trypanosoma cruzi strains in single and mixed infections: In vitro and in the intestinal tract of the blood-sucking bug, Triatoma brasiliensis. Acta tropica, v. 101, p. 225-231, 2007. | pt_BR |
| ISSN | 0001-706X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/39775 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Triatoma brasiliensis | pt_BR |
| Subject in Portuguese | Doença de Chagas | pt_BR |
| Subject in Portuguese | Infecções mistas | pt_BR |
| Title | Growth behaviour of two Trypanosoma cruzi strains in single and mixed infections: In vitro and in the intestinal tract of the blood-sucking bug, Triatoma brasiliensis | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.actatropica.2007.02.004 | |
| Abstract | Competition and cooperation are well-recognized biological phenomena, even among parasites. Co-infection of parasites in a
single host leads to several outcomes, one being competition for a limited resource. Here, the behaviour of mixed infection was
evaluated using two isolates of Trypanosoma cruzi, previously typed as belonging to genotypes TcI and TcII. The growth in vitro and
in the different compartments of the gut of Triatoma brasiliensis was studied. In vitro growth showed that MDID/BR/1999/M1 (TcI)
has a doubling time of 19.5 h and MIDID/BR/1999/JCPD4 (TcII) of 9.6 h, while the mixed infection group presented a doubling time
of 13.9 h. In vivo, three groups of infection were done: M1/TcI, JCPD4/TcII and mixed infection (50% of each strain), respectively.
All comparisons among the groups were done using the Kruskal–Wallis non-parametric test. The data showed that the in vitro culture
of mixed populations has a similar pattern to the growth of M1/TcI, apparently suggesting a positively selection for M1/TcI strain, in
axenic culture. In the gut of the insects, M1/TcI isolate and mixed infections colonized predominantly the rectalwall and rectal lumen,
in contrast to the JCPD4/TcII isolate, which was found mainly colonizing the small intestine. According to the isolates investigated,
it could be concluded that the doubling time was not determinant factor for the final composition of a co-infection. Moreover, mixed
infections resulted in a homogenous distribution of the parasites, comparing to the isolates studied separately. Apparently, in the gut
of the bugs, the simultaneous presence of JCPD4/TcII isolate resulted in an improvement of the number of parasites from M1/TcI. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Chagas disease | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Mixed infections | pt_BR |
| Subject | Triatoma brasiliensis | pt_BR |
| e-ISSN | 1873-6254 | |
| Embargo date | 2025-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
