| Author | Costa, Rafaela G. A. | |
| Author | Anunciação, Talita Andrade da | |
| Author | Araujo, Morgana de S. | |
| Author | Souza, César Alexandre de | |
| Author | Dias, Rosane Borges | |
| Author | Sales, Caroline Brandi Schlaepfer | |
| Author | Rocha, Clarissa Araújo Gurgel | |
| Author | Soares, Milena Botelho Pereira | |
| Author | Silva, Felipe Moura Araujo da | |
| Author | Koolen, Hector Henrique Ferreira | |
| Author | Costa, Emmanoel Vilaça | |
| Author | Bezerra, Daniel Pereira | |
| Access date | 2020-02-07T12:22:56Z | |
| Available date | 2020-02-07T12:22:56Z | |
| Document date | 2020 | |
| Citation | COSTA, Rafaela G. A. et al. In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil. Biomedicine & Pharmacotherapy, v. 122, p. 1-10, 2020. | pt_BR |
| ISSN | 0753-3322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/39794 | |
| Sponsorship | Brazilian agencies
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
(CAPES), Conselho Nacional de Desenvolvimento Científico e
Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do
Amazonas (FAPEAM) and Fundação de Amparo à Pesquisa do Estado da
Bahia (FAPESB). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Guatteria megalophylla | pt_BR |
| Subject in Portuguese | Óleo essencial | pt_BR |
| Subject in Portuguese | Spathulenol | pt_BR |
| Subject in Portuguese | Anti-leucemia | pt_BR |
| Title | In vitro and in vivo growth inhibition of human acute promyelocytic leukemia HL-60 cells by Guatteria megalophylla Diels (Annonaceae) leaf essential oil | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.biopha.2019.109713 | |
| Abstract | Guatteria megalophylla Diels (Annonaceae) is an 8-10 m tall tree that grows near streams and is widely spread throughout Colombian, Ecuadorian, Peruvian, Brazilian and Guianese Amazon rainforest. Herein, we investigated for the first time the chemical composition and in vitro and in vivo anti-leukemia potential of G. megalophylla leaf essential oil (EO) using human promyelocytic leukemia HL-60 cells as model. EO was obtained by a hydrodistillation clevenger-type apparatus and characterized quali- and quantitatively by GC-MS and GC-FID, respectively. In vitro cytotoxic potential of EO was evaluated in human cancer cell lines (HL-60, MCF-7 CAL27, HSC-3, HepG2 and HCT116) and in human non-cancer cell line (MRC-5) by Alamar blue method. Annexin V/propidium iodide staining, cell cycle distribution and reactive oxygen species (ROS) were assessed by flow cytometry for HL-60 cells treated with EO. In vivo efficacy of EO (50 and 100 mg/kg) was evaluated in C.B-17 SCID mice with HL-60 cell xenografts. Chemical composition analyses showed spathulenol, γ-muurolene, bicyclogermacrene, β-elemene and δ-elemene as main constituents of assayed sample. EO displayed in vitro cytotoxicity, including anti-leukemia effect with IC50 value of 12.51 μg/mL for HL-60 cells. EO treatment caused augment of phosphatidylserine externalization and DNA fragmentation without increasing of ROS in HL-60 cells. In vivo tumor mass inhibition rates of EO was 16.6-48.8 %. These data indicate anti-leukemia potential of G. megalophylla leaf EO. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil. | pt_BR |
| Affilliation | Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Federal University of Bahia. Institute of Health Sciences. Department of Biomorphology. Salvador, BA, Brazil.. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil. | pt_BR |
| Affilliation | Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil. | pt_BR |
| Affilliation | Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Subject | Guatteria megalophylla | pt_BR |
| Subject | Essential oil | pt_BR |
| Subject | Spathulenol | pt_BR |
| Subject | Anti-leukemia | pt_BR |
