| Author | Nakatani, Sueli M. | |
| Author | Santos, Carlos A. | |
| Author | Riediger, Irina Nastassja | |
| Author | Krieger, Marco Aurélio | |
| Author | Duarte, Cesar A. B. | |
| Author | Debur, Maria do Carmo | |
| Author | Carrilho, Flair J. | |
| Author | Ono, Suzane K. | |
| Access date | 2020-02-11T19:48:16Z | |
| Available date | 2020-02-11T19:48:16Z | |
| Document date | 2011 | |
| Citation | NAKATANI, Sueli M. et al. Comparative performance evaluation of hepatitis C virus genotyping based on the 5’ untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C. Virology Journal, v. 8, n. 459, p. 1-5, 2011. | pt_BR |
| ISSN | 1743-422X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/39884 | |
| Language | por | pt_BR |
| Publisher | BMC | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Hepacivirus | pt_BR |
| Title | Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1186/1743-422X-8-459 | |
| Abstract | Genotyping of hepatitis C virus (HCV) has become an essential tool for prognosis and prediction of treatment duration. The aim of this study was to compare two HCV genotyping methods: reverse hybridization line probe assay (LiPA v.1) and partial sequencing of the NS5B region. As methods: Plasma of 171 patients with chronic hepatitis C were screened using both a commercial method (LiPA HCV Versant, Siemens, Tarrytown, NY, USA) and different primers targeting the NS5B region for PCR amplification and sequencing analysis. And as results: comparison of the HCV genotyping methods showed no difference in the classification at the genotype level. However, a total of 82/171 samples (47.9%) including misclassification, non-subtypable, discrepant and inconclusive results were not classified by LiPA at the subtype level but could be discriminated by NS5B sequencing. Of these samples, 34 samples of genotype 1a and 6 samples of genotype 1b were classified at the subtype level using sequencing of NS5B. It has as conclusions that: Sequence analysis of NS5B for genotyping HCV provides precise genotype and subtype identification and an accurate epidemiological representation of circulating viral strains. | pt_BR |
| Affilliation | Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil / Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Centro de Genomas, São Paulo, São Paulo, Brasil. | pt_BR |
| Affilliation | Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil. | pt_BR |
| Subject | Hepatitis C virus | pt_BR |
| Subject | Genotyping Techniques | pt_BR |
| Subject | 5' Untranslated Regions | pt_BR |
| Subject in Spanish | Técnicas de Genotipaje | pt_BR |
| Subject in Spanish | Regiones no Traducidas 5' | pt_BR |
| DeCS | Técnicas de Genotipagem | pt_BR |
| DeCS | Regiões 5' não Traduzidas | pt_BR |
| e-ISSN | 1743-422X | |
