| Author | Costa, Sidnei Ferro | |
| Author | Gomes, Vinícius Oliveira | |
| Author | Maciel, Marilene Oliveira dos Santos | |
| Author | Melo, Larissa Martins | |
| Author | Venturin, Gabriela Lovizutto | |
| Author | Bragato, Jaqueline Poleto | |
| Author | Rebech, Gabriela Torres | |
| Author | Santos, Catiule de Oliveira | |
| Author | Oliveira, Bárbara Maria Nascimento de | |
| Author | Oliveira, Geraldo Gileno de Sá | |
| Author | Lima, Valéria Marçal Felix de | |
| Access date | 2020-02-12T12:40:38Z | |
| Available date | 2020-02-12T12:40:38Z | |
| Document date | 2020 | |
| Citation | COSTA, Sidnei Ferro et al. Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis. PLoS Neglected Tropical Diseases, p. 1-21, 21 Jan. 2020. | |
| ISSN | 1935-2727 | |
| URI | https://www.arca.fiocruz.br/handle/icict/39899 | |
| Sponsorship | São Paulo Research Foundation (FAPESP), process #2017/10906-8 | |
| Sponsorship | National Council for Scientific and Technological Development (CNPq), process #400063/2016-6 | |
| Sponsorship | CNPq/INCT-DT, process #573839/2008-5 | |
| Sponsorship | CNPq-PROEP, process #400913/2013-5 | |
| Sponsorship | Coordination for the Improvement of Higher Education Personnel (CAPES) - Finance Code 001 | |
| Language | eng | en_US |
| Publisher | Public Library of Science | |
| Later version | https://www.arca.fiocruz.br/handle/icict/40815 | |
| Rights | open access | |
| Subject in Portuguese | Leishmaniose visceral | pt_BR |
| Subject in Portuguese | IL-12 | pt_BR |
| Subject in Portuguese | IL-15 | pt_BR |
| Subject in Portuguese | Leishmania infantum | pt_BR |
| Subject in Portuguese | Imunidade celular | pt_BR |
| Title | Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis | en_US |
| Type | Preprint | |
| DOI | 10.1371/journal.pntd.0008021 | |
| Abstract | Domestic dogs are the main reservoir of Leishmania infantum, a causative agent of visceral leishmaniasis (VL). The number of human disease cases is associated with the rate of canine infection. Currently available drugs are not efficient at treating canine leishmaniasis (CanL) and months after the treatment most dogs show disease relapse, therefore the development of new drugs or new therapeutic strategies should be sought. In CanL, dogs lack the ability to mount a specific cellular immune response suitable for combating the parasite and manipulation of cytokine signaling pathway has the potential to form part of effective immunotherapeutic methods. In this study, recombinant canine cytokines (rcaIL-12, rcaIL-2, rcaIL-15 and rcaIL-7) and soluble receptor IL-10R1 (rcasIL-10R1), with antagonistic activity, were evaluated for the first time in combination (rcaIL-12/rcaIL-2, rcaIL-12/rcaIL-15, rcaIL-12/rcasIL-10R1, rcaIL-15/rcaIL-7) or alone (rcasIL-10R1) to evaluate their immunomodulatory capacity in peripheral blood mononuclear cells (PBMC) from dogs with leishmaniasis. All the combinations of recombinant proteins tested were shown to improve lymphoproliferative response. Further, the combinations rcaIL-12/rcaIL-2 and rcaIL- 12/rcaIL-15 promoted a decrease in programmed cell death protein 1 (PD-1) expression in lymphocytes. These same combinations of cytokines and rcaIL-12/rcasIL-10R1 induced IFN-γ and TNF-α production in PBMC. Furthermore, the combination IL-12/IL-15 led to an increased in T-bet expression in lymphocytes. These findings are encouraging and indicate the use of rcaIL-12 and rcaIL-15 in future in vivo studies aimed at achieving polarization of cellular immune responses in dogs with leishmaniasis, which may contribute to the development of an effective treatment against CanL. | en_US |
| Affilliation | Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil. | |
| Affilliation | Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil. | |
| Affilliation | Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil. | |
| Affilliation | Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil. | |
| Affilliation | Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil. | |
| Affilliation | Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil. | |
| Affilliation | Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | |
| Affilliation | Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil. | |
| Subject | Visceral leishmaniasis | en_US |
| Subject | Il-12 | en_US |
| Subject | IL-15 | en_US |
| Subject | Leishmania infantum | en_US |
| Subject | Immunity Cellular | en_US |
| e-ISSN | 1935-2735 | |
| Peer reviewed | Não | |
| Publication status | Publicado | |
