| Author | Senegaglia, Alexandra Cristina | |
| Author | Brofman, Paulo Roberto Slud | |
| Author | Aita, Carlos Alberto Mayora | |
| Author | Dallagiovanna, Bruno | |
| Author | Rebelatto, Carmen Lúcia Kuniyoshi | |
| Author | Hansen, Paula | |
| Author | Barchiki, Fabiane | |
| Author | Krieger, Marco Aurélio | |
| Access date | 2020-02-12T16:06:13Z | |
| Available date | 2020-02-12T16:06:13Z | |
| Document date | 2008 | |
| Citation | SENEGAGLIA, Alexandra Cristina et al. In vitro formation of capillary tubules from human umbilical cord blood cells with perspectives for therapeutic application. Revista Brasileira de Cirurgia Cardiovascular, v. 23, n. 4, p. 467-473, 2008. | pt_BR |
| ISSN | 1678-9741 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/39914 | |
| Language | por | pt_BR |
| Publisher | Sociedade Brasileira de Cirurgia Cardiovascular | pt_BR |
| Rights | open access | pt_BR |
| Title | In vitro formation of capillary tubules from human umbilical cord blood cells with perspectives for therapeutic application | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/s0102-76382008000400003 | |
| Abstract | Endothelial progenitor cells (EPC) caracterized by the CD133+ marker contribute to neovascularization. Increasing EPC number in vitro could be a promising therapeutic tool. Human umbilical cord blood maintains a significant number of EPCs, suggesting the possibility of using these cells to induce revascularization of ischemic tissues. The aim of this study was to analyze the in-vitro function of differentiated CD133+ cells. As method has that, Cells were characterized by flow cytometry; VEGF mRNA expression was evaluated by RT-PCR analysis and the functionally by essays of capillary tubes formation. It resulted that, differentiated cells lost EPC markers, maintained low levels of markers for hematopoietic and monocytic cell lines and increased the expression of adult endothelial cell markers. Differentiated cells expressed VEGF mRNA and were capable to induce in vitro capillary tubules formation. Then it concluded that, CD133+ cells differentiated into endothelial cells in vitro are functionally active initiating the possibility of their use in future therapeutic applications. | pt_BR |
| Affilliation | Pontifícia Universidade Católica do Paraná. Centro de Cardiomioplastia Celular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Pontifícia Universidade Católica do Paraná. Centro de Cardiomioplastia Celular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Pontifícia Universidade Católica do Paraná. Centro de Cardiomioplastia Celular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Pontifícia Universidade Católica do Paraná. Centro de Cardiomioplastia Celular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Pontifícia Universidade Católica do Paraná. Centro de Cardiomioplastia Celular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Pontifícia Universidade Católica do Paraná. Centro de Cardiomioplastia Celular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Subject | Endothelial Cells | pt_BR |
| Subject | Cell Proliferation | pt_BR |
| Subject | Cord Blood Stem Cell Transplantation | pt_BR |
| Subject | Neovascularization Physiologic | pt_BR |
| Subject in Spanish | Células Endoteliales | pt_BR |
| Subject in Spanish | Proliferación Celular | pt_BR |
| Subject in Spanish | Trasplante de Células Madre de Sangre del Cordón Umbilical | pt_BR |
| Subject in Spanish | Neovascularización Fisiológica | pt_BR |
| DeCS | Células Endoteliais | pt_BR |
| DeCS | Proliferação de Células | pt_BR |
| DeCS | Transplante de Células-Tronco de Sangue do Cordão Umbilical | pt_BR |
| DeCS | Neovascularização Fisiológica | pt_BR |
| e-ISSN | 1678-9741 | |
