| Author | Santos, Camila Ramos dos | |
| Author | Fessel, Melissa Regina | |
| Author | Vieira, Leandro de Carvalho | |
| Author | Krieger, Marco Aurélio | |
| Author | Goldenberg, Samuel | |
| Author | Guimarães, Beatriz Gomes | |
| Author | Zanchin, Nilson Ivo Tonin | |
| Author | Barbosa, João Alexandre Ribeiro Gonçalves | |
| Access date | 2020-02-20T14:22:52Z | |
| Available date | 2020-02-20T14:22:52Z | |
| Document date | 2009 | |
| Citation | SANTOS, Camila Ramos dos et al. Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, v. 6, n. 6, p. 1-9, 2011. | pt_BR |
| ISSN | 1744-3091 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40105 | |
| Language | por | pt_BR |
| Publisher | International Union of Crystallography | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Title | Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1107/S1744309109017734 | |
| Abstract | Q4DV70 is annotated in the Trypanosoma cruzi CL Brener genome as a hypothetical protein with a predicted thioredoxin-like fold, although the catalytic cysteine residues that are conserved in typical oxidoreductases are replaced by serine residues. Gene-expression analysis indicates that this protein is differentially expressed during the T. cruzi life cycle, suggesting that it plays an important role during T. cruzi development. The gene coding for Q4DV70 was cloned and the protein was overexpressed in Escherichia coli with an N-terminal His tag. Purification of Q4DV70 was carried out by affinity and size-exclusion chromatography and the His tag was removed by TEV protease digestion. Crystals of Q4DV70 were grown using the sitting-drop vapour-diffusion method. A diffraction data set was collected to 1.50 A resolution from a single crystal grown in 25% PEG 1500, 200 mM sodium thiocyanate pH 6.9, 10 mM phenol and 10% ethylene glycol. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 35.04, b = 50.32, c = 61.18 A. The Q4DV70 structure was solved by molecular replacement using protein disulfide isomerase from yeast (PDB code 2b5e) as a search model. Initial refinement of the model indicated that the solution was correct. These data are being used for refinement of the model of Q4DV70. | pt_BR |
| Affilliation | Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil / Universidade Estadual de Campinas. Instituto de Biologia. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Synchrotron SOLEIL. Saint-Aubin, France. | pt_BR |
| Affilliation | Universidade Estadual de Campinas. Instituto de Biologia. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Campinas. Instituto de Biologia. Campinas, SP, Brasil. | pt_BR |
| Subject | Thioredoxins | pt_BR |
| Subject | Gene Expression | pt_BR |
| Subject | Genetic Structures | pt_BR |
| Subject in Spanish | Expresión Génica | pt_BR |
| Subject in Spanish | Estructuras Genéticas | pt_BR |
| DeCS | Tiorredoxinas | pt_BR |
| DeCS | Expressão Gênica | pt_BR |
| DeCS | Estruturas Genéticas | pt_BR |
| e-ISSN | 1744-3091 | |
