| Author | Bombaça, Ana Cristina Souza | |
| Author | Brunoro, Giselle Villa Flor | |
| Author | Lopes, Geovane Dias | |
| Author | Ennes-Vidal, Vitor | |
| Author | Carvalho, Paulo Costa | |
| Author | Perales, Jonas | |
| Author | d`Avila-Levy, Claudia M. | |
| Author | Valente, Richard Hemmi | |
| Author | Menna-Barreto, Rubem F. S. | |
| Access date | 2020-04-02T18:54:15Z | |
| Available date | 2020-04-02T18:54:15Z | |
| Document date | 2020 | |
| Citation | BOMBAÇA, Ana Cristina et al. Glycolytic profile shift and antioxidant triggering in symbiont-free and H2O2-resistant Strigomonas culicis. Free Radical Biology and Medicine, v. 146, p. 392-401, 2020. | pt_BR |
| ISSN | 0891-5849 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40623 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Metabolismo energético | pt_BR |
| Subject in Portuguese | Via de pentose fosfato | pt_BR |
| Subject in Portuguese | Estresse oxidativo | pt_BR |
| Subject in Portuguese | Sistema antioxidante | pt_BR |
| Subject in Portuguese | Ascorbato peroxidase | pt_BR |
| Title | Glycolytic profile shift and antioxidant triggering in symbiont-free and H2O2-resistant Strigomonas culicis | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.freeradbiomed.2019.11.025 | |
| Abstract | During their life cycle, trypanosomatids are exposed to stress conditions and adapt their energy and antioxidant
metabolism to colonize their hosts. Strigomonas culicis is a monoxenous protist found in invertebrates with an
endosymbiotic bacterium that completes essential biosynthetic pathways for the trypanosomatid. Our research
group previously generated a wild-type H2O2-resistant (WTR) strain that showed improved mitochondrial metabolism and antioxidant defenses, which led to higher rates of Aedes aegypti infection. Here, we assess the
biological contribution of the S. culicis endosymbiont and reactive oxygen species (ROS) resistance to oxidative
and energy metabolism processes. Using high-throughput proteomics, several proteins involved in glycolysis and
gluconeogenesis, the pentose phosphate pathway and glutathione metabolism were identified. The results
suggest that ROS resistance decreases glucose consumption and indicate that the metabolic products from
gluconeogenesis are key to supplying the protist with high-energy and reducing intermediates. Our hypothesis
was confirmed by biochemical assays showing opposite profiles for glucose uptake and hexokinase and pyruvate
kinase activity levels in the WTR and aposymbiotic strains, while the enzyme glucose-6P 1-dehydrogenase was
more active in both strains. Regarding the antioxidant system, ascorbate peroxidase has an important role in
H2O2 resistance and may be responsible for the high infection rates previously described for A. aegypti. In
conclusion, our data indicate that the energy-related and antioxidant metabolic processes of S. culicis are
modulated in response to oxidative stress conditions, providing new perspectives on the biology of the trypanosomatid-insect interaction as well as on the possible impact of resistant parasites in accidental human infection. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados de Protozzologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Proteômica Estrutural e Computacional. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados de Protozzologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Energetic metabolism | pt_BR |
| Subject | Pentose phosphate pathway | pt_BR |
| Subject | Oxidative stress | pt_BR |
| Subject | Antioxidant system | pt_BR |
| Subject | Ascorbate peroxidase | pt_BR |
| e-ISSN | 873-4596 | |
| Embargo date | 2025-01-01 | |
